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Vegetal Range2019-05-21T11:12:06+01:00

Vegetal Media for Growth of E. Coli Cultures

Formedium has added six vegetal media to its range of culture media for growth of E. coli. In all six vegetal media, Tryptone has been replaced by Soya Peptone, for the following formulations of LB Miller medium, LB Lennox medium and 2YT medium. Tryptone and Yeast Extract are present as a general nitrogen source for growth and protein expression. Both are excellent sources for nitrogen and additionally Yeast Extract provides B-type vitamins, carbohydrates and growth factors.

In vegetal media,thus avoiding any contamination of medium with constituents of animal origin, Tryptone is replaced by Soya Peptone. In contrast to Tryptone, which is a pancreatic digest of bovine milk casein, its replacement Soya Peptone is made by Papaic hydrolysis of Soya Bean meal. Nitrogen content of Soya Peptone (9.5 %) compared to Tryptone is slightly lower and the same for its amino nitrogen content (3 %). This difference of less than two percent does not notably influence growth and protein expression.

“A comparative study between the use of Tryptone as a nitrogen source and Soya Peptone as an alternative source showed no difference in growth.”

In liquid media cell growth of E. Coli is measured by optical density at 680nm this did not show any significant variance between growth curves of cells cultured in LB Miller Broth, LB Lennox Broth and 2YT Broth medium containing Tryptone or Soya Peptone.
In solid media counts of Colony Forming Units (CFU) of LB Lennox Agar, LB Miller Agar and 2YT Agar containing Tryptone or Soya Peptone did not significantly differ from each other.

No substantial difference could be noted between media containing Soya Peptone and Tryptone. Soya Bean Peptone performed as well compared to the use of Tryptone in both liquid and solid media.

In all three media LB Miller Vegetal medium, LB Lennox Vegetal medium and 2YT Vegetal medium including Soya Peptone, no animal sourced constituents are present. Due to the absence of animal sourced components Formedium certifies;

  • No raw material sourced or derived from animals are used in the manufacture of this product.
  • The materials used in the production are in compliance with the European directive 75 / 318 / EEC as amended by Directive 1999 / 82 / EC.

Therefore Formedium Ltd. takes the position that this product is free of any risk in terms of Bovine Spongiform Encephalothy (BSE) or Transmissible Spongiform Encephalopathy (TSE).

Formedium Vegetal media

Methods:
Broth culture: Cell growth was measured in LB Lennox, LB Miller and 2YT medium.
Each medium was present in a formulation containing Tryptone or Soya Peptone.

  • Single colony incubated in 5 ml of all three media tested (3 Tryptone vs 3 Soya Bean peptone) O/N @ 37 °C 180 rpm.
  • Following day 1:100 dilution, 30 ml culture in 150 ml flask incubated @ 37 °C 180 rpm. Growth measured by optical density at 680nm, measured hourly
  • 3 biological repeats
  • LB Lennox (g/l); Soya peptone 10 or Tryptone 10, Yeast extract 5, NaCl 5.
    LB Miller (g/l); Soya peptone 10 or Tryptone 10, Yeast extract 5, NaCl 10.
    2YT; (g/l); Soya peptone 16 or Tryptone 16, Yeast extract 10, NaCl 5.

Formedium Vegetal media

Methods:
Agar culture on plates: The number of Colony Forming units was measured in LB Lennox, LB Miller and 2YT Agar plates.
Each medium was present in a formulation containing Tryptone or Soya Peptone.

  • Single colony incubated in 5 ml of all three media tested (3 Tryptone vs 3 Soya Bean peptone) O/N @ 37 °C 180 rpm.
  • Following day 100 µl of 10-fold dilution series (to 10-6) was spread onto the same medium as agar (e.g., Miller broth colony onto Miller agar, Lennox-Soya colony onto Lennox-Soya agar). Incubated O/N @ 37 °C
  • Colony count (cfu/ml) for 3 biological repeats
  • LB Lennox (g/l); Soya peptone 10 or Tryptone 10, Yeast extract 5, NaCl 5, Agar 15.
    LB Miller (g/l); Soya peptone 10 or Tryptone 10, Yeast extract 5, NaCl 10, Agar 15.
    2YT; (g/l); Soya peptone 16 or Tryptone 16, Yeast extract 10, NaCl 5, Agar 15.
  • Soya Peptone

    £19.20£320.00
    SKU Pack Size Price
    VPEP01250g £19.20
    VPEP02500g £33.40
    VPEP031000g £58.00
    VPEP046 x 1kg £320.00
    Soya Peptone is a papaic digest of defatted soybean flour and is a well-balanced source of essential amino acids, carbohydrates and vitamins in cell cultures.Soya Peptone is used for growth of a wide variety of bacteria and yeasts in cell cultures and is often combined with Tryptone or Peptone for a rapid and abundant growth of cells.This plant peptone is classified animal-free by Formedium Ltd. Based on the manufacturing protocol, we attest that no animal raw materials are prescribed for use in the production of this product, nor are any of the raw materials derived from animal products.Soya Peptone is classified animal free, GMO free (according to the European Directive 2001/18/CE)Store dry at room temperature
  • VLB-Agar Lennox

    £2.79£200.12
    SKU Pack Size Price
    VLBAL1L1 Litre pack £2.79
    VLBAL2L2 Litre pack £4.23
    VLBAL5L5 Litre pack £8.24
    VLBAL10L10L pack £14.03
    VLBAL20L20 Litre pack £26.27
    VLBAL0250250g £10.91
    VLBAL0500500g £21.60
    VLBAL10001000g £37.18
    VLBAL60006 x 1kg £200.12
    Select options
    Formula
    g/l
    Soya Peptone10
    Yeast extract5
    NaCl5
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 35 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • VLB-Broth Lennox

    £2.56£167.40
    SKU Pack Size Price
    VLBBL1L1 Litre pack £2.56
    VLBBL2L-12 Litre pack £4.12
    VLBBL5L5 Litre pack £5.90
    VLBBL10L10L pack £10.24
    VLBBL20L20 Litre pack £16.37
    VLBBL0250250g £10.80
    VLBBL0500500g £19.59
    VLBBL10001kg £35.73
    VLBBL60006 x 1kg £167.40
    Select options
    Formula
    g/l
    Soya Peptone10
    Yeast extract5
    NaCl5
    Final pH7.0 ± 0.2 at 25°C
    Suspend 20 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • VLB-Agar Miller

    £3.23£265.57
    SKU Pack Size Price
    VLBAM1L1 Litre pack £3.23
    VLBAM2L2 Litre pack £4.90
    VLBAM5L5 Litre pack £11.47
    VLBAM10L10L pack £21.04
    VLBAM20L20 Litre pack £39.74
    VLBAM0250250g £15.48
    VLBAM0500500g £28.28
    VLBAM10001000g £49.09
    VLBAM60006 x 1kg £265.57
    Select options
    Formula
    g/l
    Soya Peptone10
    Yeast extract5
    NaCl10
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 40 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • VLB-Broth Miller

    £2.56£185.88
    SKU Pack Size Price
    VLBBM1L1 Litre pack - 25g £2.56
    VLBBM2L2 Litre pack - 50g £3.57
    VLBBM5L5 Litre pack - 125 gram £7.02
    VLBBM10L10 Litre pack - 250g £11.91
    VLBBM20L20 Litre pack - 500g £22.26
    VLBBM0250250g £11.91
    VLBBM0500500g £22.26
    VLBBM10001000g £36.96
    VLBBM15001500g £52.65
    VLBBM60006 x 1kg £185.88
     
    Select options
    Formula
    g/l
    Soya Peptone10
    Yeast extract5
    NaCl10
    Final pH7.0 ± 0.2 at 25°C
    Suspend 25 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • 2X VYT Broth

    £3.23£244.86
    SKU Pack Size Price
    VYTB1L1 Litre pack - 31g £3.23
    VYTB2L2 Litre pack £4.90
    VYTB5L5 Litre pack £8.24
    VYTB10L10 Litre pack - 310g £15.81
    VYTB20L20 Litre pack £30.39
    VYTB0250250g £12.92
    VYTB10001000g £44.75
    VYTB60006 x 1kg £244.86
    Select options
    Formula
    g/l
    Soya Peptone16
    Yeast extract10
    NaCl5
    Final pH7.0 ± 0.2 at 25°C
    Suspend 31 gram powdered medium in 1 litre distilled water Store dry at room temperature.
    View product
  • 2X VYT Agar

    £4.23£306.41
    SKU Pack Size Price
    VYTA1L1 Litre pack £4.23
    VYTA2L2 Litre pack £6.35
    VYTA5L5 Litre pack £14.70
    VYTA10L10L pack £28.05
    VYTA20L20 Litre pack £53.76
    VYTA0250250g £15.92
    VYTA10001000g £56.66
    VYTA60006 x 1kg £306.41
    Select options
    Formula
    g/l
    Soya Peptone16
    Yeast extract10
    NaCl5
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 46 gram powdered medium in 1 litre distilled water Store dry at room temperature.
    View product