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Schizosaccharomyces Pombe Minimal Defined Media Technical Data2018-01-25T13:55:53+00:00

Schizosaccharomyces Pombe Minimal Defined Media
Technical Data

EMMEMM w/o DextroseEMM-Low GlucoseEMMS
(1,2 M Sorbitol)
EMM w/o PhosphateEMM w/o NitrogenEMM
SP
MB
Medium
MMA Agar MediumUnits
Carbon Source
Dextrose20.005.0020.0020.0020.0020.00g
Glucose5.0010.00g
Sorbitol216.00g
Amino Acids
Adenine50.00mg
Histidine50.00mg
Lysine50.00mg
L-Leucine150.00mg
Uracil150.00mg
Minerals
Phtalic Acid K+3.003.003.003.003.003.003.00g
Na2HPO42.202.202.202.202.202.20g
NH4Cl5.005.005.005.005.005.00g
MgCl2.6H2O1.051.051.051.051.051.051.05g
CaCl2.2H2O14.7014.7014.7014.7014.7014.7014.70100.00100.00mg
KCl1.001.001.001.001.001.001.00g
Na2SO440.0040.0040.0040.0040.0040.0040.00mg
KH2PO4500.001000.00mg
K+ Acetate360.00mg
MgSO4.7H20500.00500.00mg
NaCl100.00100.00mg
(NH4)2SO45000.005000.00mg
Vitamins
Panthotenic acid1.001.001.001.001.001.001.00mg
Nicotinic acid10.0010.0010.0010.0010.0010.0010.0010.0010.00mg
Inositol10.0010.0010.0010.0010.0010.0010.0010.0010.00mg
Biotin1.001.001.001.001.001.001.000.010.01mg
Ca-Pantothenate1.001.00mg
Trace Elements
H3BO30.500.500.500.500.500.500.500.500.50mg
MnSO4.H2O0.400.400.400.400.400.400.400.400.40mg
ZnSO4.7H2O0.400.400.400.400.400.400.400.400.40mg
FeCl3.6H2O0.200.200.200.200.200.200.200.200.20mg
Na2MoO4.2H2O0.040.040.040.040.040.040.040.040.04mg
KI0.100.100.100.100.100.100.100.100.10mg
CuSO4.5H2O0.040.040.040.040.040.040.040.040.04mg
Citric Acid1.001.001.001.001.001.001.00mg
Agar
Agar(17)(17)(17)(17)(17)(17)17.00g
Total
Total32.3012.3017.30248.3030.1027.3032.5011.90g/l
Total (Agar)(49.30)(23.30)(34.30)(47.10)(44.30)(49.50)g/l