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  • EDTA Disodium

    $12.47$192.31
    SKU Pack Size Price
    EDTA250 250g $12.47
    EDTA500 500g $20.79
    EDTA6000 6 x 1kg $192.31
    EDTA1000 1000g $34.65
    C10H14N2O8Na2.2H2O = 372.2
    EDTA Na2.2H2O Ethylenediaminetetra-acetate Disodium Dihydrate
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    Specifications
     
    Purity >99%
    Store dry at room temperature Soluble in water (20°C. 100g/L) Hygroscopic, protect against moisture CAS 6381-92-6
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  • MES Monohydrate

    $32.92$1,403.33
    SKU Pack Size Price
    MES02 250g $77.96
    MES03 500g $142.94
    MES05 6 x 1kg $1,403.33
    MES01 100g $32.92
    MES04 1000g $259.88
    2-(N-Morpholino) Ethanesulfonic acid C6H13NO4S·H2O=213.2
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    Specifications
     
    Appearance White Crystalline Powder
    Assay (Titration) ≥99%
    Water Content 8.0-8.9%
    pH 1% Di HB2BO 3.5-4.5
    Solubility 0.1M water Clear Colourless Solution
    Residue On Ignition ≤0.1%
    AB280B, 0.5M water ≤0.020
    AB260B, 0.5M water ≤0.025
    pKa 25 5.9-6.3
    Heavy metals (Pb) ≤5ppm
    Store dry at room temperature Soluble in water CAS 145224-94-8
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  • MOPS

    $29.11$909.56
    SKU Pack Size Price
    MOPS02 250g $43.31
    MOPS03 500g $81.43
    MOPS05 6 x 1kg $909.56
    MOPS01 100g $29.11
    MOPS04 1000g $159.39
    4-Morpholino propanesulfonic acid C7H15NO4S=209.3
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    Specifications
     
    Appearance White crystalline powder
    Assay (Titration, Dry Base) ≥99.0%
    Water Content by KF ≤1%
    Infrared Complies
    Solubility 1M water Clear colourless solution
    pH 1% Di H2O 3.6 - 4.4 (25°C)
    A280, 1M water ≤0.10
    A260, 1M water ≤0.15
    Store dry at room temperature Soluble in water CAS 1132-61-2
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  • TRIS

    $32.48$249.48
    SKU Pack Size Price
    TRIS05 500g $32.48
    TRIS01 1kg $45.05
    TRIS06 6 x 1kg $249.48
    Ultra Pure 99.9% C4H11NO3=121.1
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    Specifications
     
    Purity 99.9~101.0%
    Solubility (0.1M, Water) Clear
    APHP Color,20% Aqueous solution <10#
    Melting point 168.5~171.5
    pH-value(5%; water) 10.0~11.5
    In water insoluble <0.005%
    Abs.@280nm; 10%; 1cm; water <0.03
    Abs.@260nm; 10%; 1cm; water <0.06
    Loss on drying (105°C,3h) <0.3%
    Residue after Ignition <0.1%
    Heavy Metals <0.0005%
    Chloride (Cl) <0.0002%
    Sulfate (SO4) <0.0005%
    As (Arsenic) <0.0003%
    Ca (Calcium) <0.0005%
    Cu (Copper) <0.0005%
    Fe (Iron) <0.0001%
    Mg (Magnesium) <0.0005%
    Mn (Manganese) <0.0005%
    Pb (Lead) <0.0002%
    Zn (Zinc) <0.0005%
    Appearance White crystalline powder
    Store dry at room temperature (18-25°C) CAS 77-86-1
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  • A Medium

    $27.37$442.13
    SKU Pack Size Price
    AMD0101 250g $27.37
    AMD0103 6 x 1kg $442.13
    AMD0102 1kg $81.95
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    Formula
    g/l
    Peptone 5
    Yeast Extract 0.5
    Glucose 5
    KH2PO4 2.25
    K2HPO4 0.7
    MgSO4.anhydrous 0.25
      Suspend 13.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • AX Medium

    $27.37$442.13
    SKU Pack Size Price
    AXM0101 250g $27.37
    AXM0103 6 x 1kg $442.13
    AXM0102 1000g $81.95
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    Formula
    g/l
    Peptone 14.3
    Yeast Extract 7.15
    Glucose 18
    KH2PO4 0.49
    Na2HPO4.anhydrous 0.49
      Suspend 40.4 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Broth

    $29.11$471.59
    SKU Pack Size Price
    SMB0102 1000g $87.32
    SMB0103 6 x 1kg $471.59
    SMB0101 250g $29.11
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    Formula
    g/l
    Peptone 10
    Yeast Extract 1
    Glucose 10
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
      Suspend 24.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Agar

    $31.01$559.94
    SKU Pack Size Price
    SMA0101 250g $31.01
    SMA0103 6 x 1kg $559.94
    SMA0102 1000g $103.78
    Select options
    Formula
    g/l
    Peptone 10
    Yeast Extract 1
    Glucose 10
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgSO4.anhydrous 0.49
    Agar 17
      Suspend 41.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Broth/5

    $29.11$471.59
    SKU Pack Size Price
    SMB50103 6 x 1kg $471.59
    SMB50102 1000g $87.32
    SMB50101 250g $29.11
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    Formula
    g/l
    Peptone 2
    Yeast Extract 0.2
    Glucose 0.2
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
      Suspend 7.89 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Agar/5

    $31.01$559.94
    SKU Pack Size Price
    SMA50103 6 x 1kg $559.94
    SMA50102 1000g $103.78
    SMA50101 250g $31.01
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    Formula
    g/l
    Peptone 2
    Yeast Extract 0.2
    Glucose 2
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
    Agar 17
      Suspend 24.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • HL5 Medium without Glucose

    $27.37$584.89
    SKU Pack Size Price
    HLB0101 250g $27.37
    HLB0103 6 x 1kg $584.89
    HLB0102 1000g $103.78
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    KH2PO4 0.5
    Na2HPO4 0.5
      Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLG0101 250g $25.47
    HLG0103 6 x 1kg $461.71
    HLG0102 1000g $83.68
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    Glucose 13.5
    KH2PO4 0.5
    Na2HPO4 0.5
      Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLC0101 250g $25.47
    HLC0103 6 x 1kg $451.84
    HLC0102 1000g $83.68
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    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    Glucose 10
      Suspend 26.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLD0102 1000g $100.14
    HLD0103 6 x 1kg $552.32
    HLD0101 250g $27.37
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    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
      Suspend 16.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLE2 1000g $87.32
    HLE3 6 x 1kg $471.59
    HLE1 250g $26.33
    HL5 supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    Glucose 13.5
    KH2PO4 0.5
    Na2HPO4 0.5
    FM Vitamins and Micro-elements 0.01
      Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLF1 250g $28.24
    HLF2 1000g $105.51
    HLF3 6 x 1kg $569.82
    HL5 Medium supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dictyostelium Discoideum who require additional nutritional elements for starting up vigorous growth or protein synthesis.
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    KH2PO4 0.5
    Na2HPO4 0.5
    FM Vitamins and Micro-elements 0.01
      Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLH1 250g $26.33
    HLH3 6 x 1kg $461.71
    HLH2 1kg $85.59
    HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.
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    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    Glucose 10
    FM Vitamins and Micro-elements 0.01
      Suspend 26.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLI2 1kg $101.87
    HLI1 250g $28.24
    HLI3 6 x 1kg $562.37
    HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.
    Select options
    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    FM Vitamins and Micro-elements 0.01
      Suspend 16.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LoFlo Medium

    $58.21$550.07
    SKU Pack Size Price
    LF0501 500g $58.21
    LF6001 6 x 1kg $550.07
    LF1001 1kg $101.87
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    Formula
    mg/l
    Glucose 11000 g/l
    KH2PO4 680.45 g/l
    Casein Peptone 5000 g/l
    NH4Cl 26.8
    MgCl2 37.1
    CaCl2 1.1
    FeCl3 8.11
    Na2-EDTA 4.84
    ZnSO4 2.30
    H3BO3 1.11
    MnCl2.4H2O 0.51
    CoCl2 0.17
    CuSO5.5H2O 0.15
    (NH4)6M07O24.4H2O 0.1
      Suspend 16.8 gram powdered medium in 1 litre distilled water Store dry at room temperature Adjust to pH 6.5
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  • SKU Pack Size Price
    LFG0501 500g $58.21
    LFG6001 6 x 1kg $550.07
    LFG1001 1kg $101.87
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    Formula
    g/l
    Glucose 11
    KH2PO4 0.68
    Casein Peptone 5
    Yeast extract 0.7
    NH4Cl 26.8
    MgCl2 37.1
    CaCl2 1.1
    FeCl3 8.11
    Na2-EDTA 4.84
    ZnSO4 2.30
    H3BO3 1.11
    MnCl2.4H2O 0.51
    CoCl2 0.17
    CuSO5.5H2O 0.15
    (NH4)6M07O24.4H2O 0.1
      Suspend 17.5 gram powdered medium in 1 litre distilled water Store dry at room temperature Adjust to pH 6.5
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  • FM Minimal Medium

    $51.98$935.55
    SKU Pack Size Price
    FMM0102 1kg $173.25
    FMM0103 6 x 1kg $935.55
    FMM0101 250g $51.98
    Select options
    Formula
    mg/l
    Amino Acids
    Arg 700
    Asp 300
    Cys 200
    GluA 500
    Gly 900
    His 300
    Ile 600
    Leu 900
    Lys 900
    Met 300
    Phe 500
    Pro 800
    Thr 500
    Trp 200
    Val 700
    Vitamins
    Biotin 0.02
    Cyanocobalamin 0.01
    Folic Acid 0.2
    Lipoic Acid 0.4
    Riboflavin 0.5
    Thiamine 0.6
    Micro Elements
    Na2EDTA.2H2O 4.84
    ZnSO4 2.3
    H3BO3 1.11
    MnCl2.4H2O 0.51
    CoCl2.6H2O 0.17
    CuSO4.5H2O 0.15
    (NH4)6Mo7O24.4H2O 0.1
    Minerals
    NH4Cl 53.5
    CaCl2.2H2O 2.94
    FeCl3 16.2
    MgCl2.6H2O 81.32
    KH2PO4 870
    Carbon Source
    Glucose 10000
    Total 19334.87
      Suspend 19.3 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0202 1kg $173.25
    FMM0203 6 x 1kg $935.55
    FMM0201 250g $51.98
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    Suspend 19.3 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0502 1kg $173.25
    FMM0503 6 x 1kg $935.55
    FMM0501 250g $51.98
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    Suspend 11.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0602 1kg $173.25
    FMM0603 6 x 1kg $935.55
    FMM0601 250g $51.98
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    Suspend 11.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0302 1kg $173.25
    FMM0303 6 x 1kg $935.55
    FMM0301 250g $51.98
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    Suspend 19.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0402 1kg $173.25
    FMM0403 6 x 1kg $935.55
    FMM0401 250g $51.98
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    Suspend 17.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0702 1000g $173.25
    FMM0703 6 x 1kg $935.55
    FMM0701 250g $51.98
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    Suspend 18.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0802 1kg $173.25
    FMM0803 6 x 1kg $935.55
    FMM0801 250g $51.98
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    Suspend 18.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SIH Medium

    $51.98$935.55
    SKU Pack Size Price
    SIH0102 1kg $173.25
    SIH0103 6 x 1kg $935.55
    SIH0101 250g $51.98
    SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1.1 mM) and increased levels of Lysine (8.5 mM) and Tryptophane (1.7 mM). Concentrations of Cysteine, Glutamic acid, Methionine, Phenylalanine, Threonine are slightly increased as well in SIH. The alterations in amino acid formulation of SIH medium resulted in a more even and better amino acid utilisation. Cell density of D. discoideum rose up to levels in excess of 5 x 107 cells compared to FM medium with cell density levels of 3 x 107. Improvement of a synthetic medium for Dictyostelium discoideum, Sang-In Han, Karl Friehs and Erwin Flaschel, Process Biochemistry, 39 (8), 925 � 930, 2004. Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor. Sang-In Han, Karl Friehs and Erwin Flaschel, Process Biochemistry, 39, 585 - 589, 2004.
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    Formula
    mg/l
    Amino Acids
    Arg 700
    Asp 300
    Asp A 150
    Cys 300
    GluA 545
    Gly 900
    His 300
    Ile 600
    Leu 900
    Lys 1250
    Met 350
    Phe 550
    Pro 800
    Thr 500
    Trp 350
    Val 700
    Vitamins
    Biotin 0.02
    Cyanocobalamin 0.01
    Folic Acid 0.2
    Lipoic Acid 0.4
    Riboflavin 0.5
    Thiamine 0.6
    Micro Elements
    Na2EDTA.2H2O 4.84
    ZnSO4 2.3
    H3BO3 1.11
    MnCl2.4H2O 0.51
    CoCl2.6H2O 0.17
    CuSO4.5H2O 0.15
    (NH4)6Mo7O24.4H2O 0.1
    Minerals
    NH4Cl 53.5
    CaCl2.2H2O 2.94
    FeCl3 16.2
    MgCl2.6H2O 81.32
    KH2PO4 870
    Carbon Source
    Glucose 10000
    Total 20326
      Suspend 20.3 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SIH medium w/o NH4Cl

    $51.98$935.55
    SKU Pack Size Price
    SIH0502 1kg $173.25
    SIH0503 6 x 1kg $935.55
    SIH0501 250g $51.98
    Select options
    Suspend 20.3 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SIH medium w/o Amino acids

    $51.98$935.55
    SKU Pack Size Price
    SIH0602 1kg $173.25
    SIH0603 6 x 1kg $935.55
    SIH0601 250g $51.98
    Select options
    Suspend 11.1 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    SIH0702 1kg $173.25
    SIH0703 6 x 1kg $935.55
    SIH0701 250g $51.98
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    Suspend 11.1 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SIH medium w/o Methionine

    $51.98$935.55
    SKU Pack Size Price
    SIM0102 1kg $173.25
    SIM0103 6 x 1kg $935.55
    SIM0101 250g $51.98
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    Suspend 20.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    SIH1002 1kg $173.25
    SIH1003 6 x 1kg $935.55
    SIH1001 250g $51.98
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    Suspend 18.4 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    SIH0802 1kg $173.25
    SIH0803 6 x 1kg $935.55
    SIH0801 250g $51.98
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    Suspend 19.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    SIH0902 1kg $173.25
    SIH0903 6 x 1kg $935.55
    SIH0901 250g $51.98
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    Suspend 18.6 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    AIMLB0110 1000g $83.68
    AIMLB0105 500g $50.24
    AIMLB0160 6 x 1kg $451.84
    AIMLB0101 100g $11.95
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    (NH4)2SO4 3.3
    KH2PO4 6.8
    Na2HPO4 7.1
    Glucose 0.5
    α-Lactose 2.0
    MgSO4 0.15
      Suspend 34.85 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    AIM2YT0110 1000g $83.68
    AIM2YT0105 500g $50.24
    AIM2YT0160 6 x 1kg $451.84
    AIM2YT0101 100g $11.95
    Select options
    Formula
    g/l
    Tryptone 16
    Yeast extract 10
    (NH4)2SO4 3.3
    KH2PO4 6.8
    Na2HPO4 7.1
    Glucose 0.5
    α-Lactose 2.0
    MgSO4 0.15
    Suspend 45.85 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    AIM2YT0210 1000g $87.32
    AIM2YT0205 500g $52.32
    AIM2YT0260 6 x 1kg $471.59
    AIM2YT0201 100g $12.82
    Select options
    Formula
    g/l
    Tryptone 16
    Yeast extract 10
    (NH4)2SO4 3.3
    KH2PO4 6.8
    Na2HPO4 7.1
    Glucose 0.5
    α-Lactose 2.0
    MgSO4 0.15
    Trace Elements 0.03
    Suspend 45.85 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LB Broth w/o NaCl

    $21.31$438.50
    SKU Pack Size Price
    LBO0101 250g $21.31
    LBO0103 6 x 1kg $438.50
    LBO0102 1000g $79.52
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 15 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LB Agar w/o NaCl

    $24.08$515.25
    SKU Pack Size Price
    LBO0201 250g $24.08
    LBO0203 6 x 1kg $515.25
    LBO0202 1000g $93.38
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 30 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • L-Broth

    $22.70$317.91
    SKU Pack Size Price
    LBL0101 250g $22.70
    LBL0103 6 x 1kg $317.91
    LBL0102 1000g $58.21
    Luria Broth Base (Miller�s Modification)
    Luria Broth Base or L-Broth is based upon the Luria Broth formulation as originally developed by Miller for cultivation and maintenance of E. coli cells in molecular biology. L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize, support a luxurious growth of E. coli cells. Sodium ions for transport and osmotic balance are provided by Sodium chloride. The concentration of NaCl in L-Broth is low compared to both LB Miller and LB Lennox formulations, respectively 10% and 5% of the NaCl concentration is present in both formulations. These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain. Miller, J.H., Experiments in molecular genetics, Cold Spring harbour Laboratory, Cold Spring harbour, New York, (1972). Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955). Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
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    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 0.5
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 15.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • L-Broth Agar

    $24.08$515.25
    SKU Pack Size Price
    LBL0201 250g $24.08
    LBL0203 6 x 1kg $515.25
    LBL0202 1000g $93.90
    Luria Agar Base (Miller's Modification)
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    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 0.5
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 30.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LB-Broth Lennox

    $3.98$260.57
    SKU Pack Size Price
    LBX0102 1kg $55.61
    LBX0103 6 x 1kg $260.57
    LBX1L 1 Litre pack $3.98
    LBX2L 2 Litre pack $6.41
    LBX5L 5 Litre pack $9.18
    LBX10L 10L pack $15.94
    LBX20L 20 Litre pack $25.47
    LBX0104 500g $30.49
    LBX0101 250g $16.81
    LB-Broth Low Salt, Lennox L Broth

    LB Broth Lennox is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize,  support a luxurious growth of E. coli cells.

    Sodium ions for transport and osmotic balance are provided by Sodium chloride. The concentration of NaCl in LB Lennox is half the concentration of the LB Miller Broth formulation and ten times the concentration of LB Luria Broth formulation. These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain.

    Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955).
    Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).

    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 20 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LB-Agar Lennox

    $4.33$311.50
    SKU Pack Size Price
    LBXA1L 1 Litre pack $4.33
    LBXA20L 20 Litre pack $40.89
    LBX0201 250g $16.98
    LBXA2L 2 Litre pack $6.58
    LBXA5L 5 Litre pack $12.82
    LBX0203 6 x 1kg $311.50
    LBXA10L 10L pack $21.83
    LBX0202 1000g $57.87
    LB-Agar Low Salt, Lennox L Agar
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 35 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LB-Top Agar Lennox

    $16.46$302.32
    SKU Pack Size Price
    LBX0301 250g $16.46
    LBX0303 6 x 1kg $302.32
    LBX0302 1000g $55.96
    LB-Top Agar Low Salt, Lennox L
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    Agar 7
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 27 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LBM-Broth Lennox

    $16.11$252.95
    SKU Pack Size Price
    LXM0101 250g $16.11
    LXM0103 6 x 1kg $252.95
    LXM0102 1000g $54.05
    LBM-Broth Low Salt
    For propagation of of lambda phages in Escherichia coli. In LBM media Mg2 is added to enhance adsorbtion of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption.
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.98
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 21 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LBM-Agar Lennox

    $16.46$302.32
    SKU Pack Size Price
    LXM0201 250g $16.46
    LXM0203 6 x 1kg $302.32
    LXM0202 1000g $55.96
    LBM-Agar Low Salt
    For propagation of of lambda phages in Escherichia coli. In LBM media Mg2 is added to enhance adsorbtion of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption.
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.98
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 36 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LB-Broth Miller

    $3.98$289.33
    SKU Pack Size Price
    LMM10L 10 Litre pack - 250g $18.54
    LMM1L 1 Litre pack - 25g $3.98
    LMM20L 20 Litre pack - 500g $34.65
    LMM0101 250g $18.54
    LMM2L 2 Litre pack - 50g $5.54
    LMM5L 5 Litre pack - 125 gram $10.91
    LMM0103 6 x 1kg $289.33
    LMM0104 500g $34.65
    LMM0105 1500g $81.95
    LMM0102 1000g $57.52
    LB-Broth High Salt, Miller's LB Broth, Luria Broth
    LB Broth Miller is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize,  support a luxurious growth of E. coli cells. Sodium ions for transport and osmotic balance are provided by Sodium chloride. The concentration of NaCl in LB Miller is twice the concentration of the LB Lennox Broth formulation and twenty times the concentration of LB Luria Broth formulation. These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain. Luria, S.E. and J.W. Burrous, Hybridization between Escherich coli and Shigella, J. Bacteriol., 74, 461-476. 1955. Luria, S.E., J.N. Adams and R.C. Ting, Transduction of lactose-utilizing ability amongst strains of E. coli and S. dysenteria and the properties of transducing phage particles, Virology, 12, 348-390, 1960. Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955). Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y. Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 10
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 25 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • LB-Agar Miller

    $5.02$413.37
    SKU Pack Size Price
    LMM02-10L 10L pack $32.74
    LMM02-1L 1 Litre pack $5.02
    LMM02-20L 20 Litre pack $61.85
    LMM0201 250g $24.08
    LMM02-2L 2 Litre pack $7.62
    LMM02-5L 5 Litre pack $17.84
    LMM0203 6 x 1kg $413.37
    LMM0204 500g $44.01
    LMM0202 1000g $76.40
    LB-Agar High Salt, Miller's LB Broth, Luria Agar
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 10
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 40 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product