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  • EDTA Disodium

    $12.47$192.31
    SKU Pack Size Price
    EDTA250 250g $12.47
    EDTA500 500g $20.79
    EDTA6000 6 x 1kg $192.31
    EDTA1000 1000g $34.65
    C10H14N2O8Na2.2H2O = 372.2
    EDTA Na2.2H2O Ethylenediaminetetra-acetate Disodium Dihydrate
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    Specifications
     
    Purity >99%
    Store dry at room temperature Soluble in water (20°C. 100g/L) Hygroscopic, protect against moisture CAS 6381-92-6
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  • HEPES

    $25.99$727.65
    SKU Pack Size Price
    HEPES10 1000g $129.94
    HEPES03 250g $41.58
    HEPES05 500g $74.50
    HEPES60 6kg $727.65
    HEPES01 100g $25.99
    C8H18N2O4S=238.3
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    Specifications
     
    HEPES >99.5%
    Loss on Drying (105 C° 4hr) <0.5%
    Water Content (By KF) <0.5%
    Infrared Complies
    pH 1% in water 4.8-5.6 (25°C)
    Solubility 1M in water Clear and colourless solution
    Residue on ignition <0.1%
    A260, 1M in water <0.045
    A280, 1M in water <0.035
    A420, 1M in water <0.20
    Heavy Metals <5 ppm
    pKa at 20°C 7.35-7.69
    Store dry at room temperature Soluble in water CAS 7365-45-9
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  • Hydroxyurea

    $142.07$1,115.73
    SKU Pack Size Price
    HDU0250 250g $1,115.73
    HDU0100 100g $485.10
    HDU0005 5g $183.83
    HDU0025 25g $142.07
    DNA replication inhibitor causing deoxyribonucleotide depletion CH4N2O2=76.05
    Hydroxyurea is an anti-neoplastic which Inactivates ribonucleoside reductase by forming a free radical nitroxide that binds a tyrosyl free radical in the active site of the enzyme. This blocks the synthesis of deoxynucleotides, which inhibits DNA synthesis and induces synchronization or cell death in S-phase.
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    Specifications
     
    Appearance White Crystalline Powder
    Content by HPLC >97.5
    Water >0.5%
    Related substances >0.2%
    Residue on ignition >0.1%
    Heavy metals >10ppm
    Residue on ignition >0.1%
    Store at 2 -8°C in dark, dry place CAS 127-07-1
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  • IPTG Dioxane free

    $32.48$883.58
    SKU Pack Size Price
    IPTG100 100g $436.59
    IPTG025 25g $136.43
    IPTG250 250g $883.58
    IPTG005 5g $32.48
    IPTG010 10g $58.47
    Isopropyl-β-D-1-Thiogalactopyranside C9H18O5S=238.3
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    Specifications
     
    Purity (HPLC) 99.9%
    Purity (TLC) One Spot
    IR Conform standard
    Solubility (5%, H2O) Clear and colourless solution
    pH (5%, H2O) 6.0
    Optical rotation (C=1, H2O) -32.4
    Melting point 111.5 - 112.4°C
    Water KF 0.1%
    Dioxane (GLC)* <1 ppm
    * Not used in manufacturing process
    Store dry at 2-8°C Soluble in ethanol and water CAS 367-93-1
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  • MES Monohydrate

    $32.92$1,403.33
    SKU Pack Size Price
    MES02 250g $77.96
    MES03 500g $142.94
    MES05 6 x 1kg $1,403.33
    MES01 100g $32.92
    MES04 1000g $259.88
    2-(N-Morpholino) Ethanesulfonic acid C6H13NO4S·H2O=213.2
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    Specifications
     
    Appearance White Crystalline Powder
    Assay (Titration) ≥99%
    Water Content 8.0-8.9%
    pH 1% Di HB2BO 3.5-4.5
    Solubility 0.1M water Clear Colourless Solution
    Residue On Ignition ≤0.1%
    AB280B, 0.5M water ≤0.020
    AB260B, 0.5M water ≤0.025
    pKa 25 5.9-6.3
    Heavy metals (Pb) ≤5ppm
    Store dry at room temperature Soluble in water CAS 145224-94-8
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  • MOPS

    $29.11$909.56
    SKU Pack Size Price
    MOPS02 250g $43.31
    MOPS03 500g $81.43
    MOPS05 6 x 1kg $909.56
    MOPS01 100g $29.11
    MOPS04 1000g $159.39
    4-Morpholino propanesulfonic acid C7H15NO4S=209.3
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    Specifications
     
    Appearance White crystalline powder
    Assay (Titration, Dry Base) ≥99.0%
    Water Content by KF ≤1%
    Infrared Complies
    Solubility 1M water Clear colourless solution
    pH 1% Di H2O 3.6 - 4.4 (25°C)
    A280, 1M water ≤0.10
    A260, 1M water ≤0.15
    Store dry at room temperature Soluble in water CAS 1132-61-2
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  • A Medium

    $27.37$442.13
    SKU Pack Size Price
    AMD0101 250g $27.37
    AMD0103 6 x 1kg $442.13
    AMD0102 1kg $81.95
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    Formula
    g/l
    Peptone 5
    Yeast Extract 0.5
    Glucose 5
    KH2PO4 2.25
    K2HPO4 0.7
    MgSO4.anhydrous 0.25
      Suspend 13.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • AX Medium

    $27.37$442.13
    SKU Pack Size Price
    AXM0101 250g $27.37
    AXM0103 6 x 1kg $442.13
    AXM0102 1000g $81.95
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    Formula
    g/l
    Peptone 14.3
    Yeast Extract 7.15
    Glucose 18
    KH2PO4 0.49
    Na2HPO4.anhydrous 0.49
      Suspend 40.4 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Broth

    $29.11$471.59
    SKU Pack Size Price
    SMB0102 1000g $87.32
    SMB0103 6 x 1kg $471.59
    SMB0101 250g $29.11
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    Formula
    g/l
    Peptone 10
    Yeast Extract 1
    Glucose 10
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
      Suspend 24.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Agar

    $31.01$559.94
    SKU Pack Size Price
    SMA0101 250g $31.01
    SMA0103 6 x 1kg $559.94
    SMA0102 1000g $103.78
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    Formula
    g/l
    Peptone 10
    Yeast Extract 1
    Glucose 10
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgSO4.anhydrous 0.49
    Agar 17
      Suspend 41.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Broth/5

    $29.11$471.59
    SKU Pack Size Price
    SMB50103 6 x 1kg $471.59
    SMB50102 1000g $87.32
    SMB50101 250g $29.11
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    Formula
    g/l
    Peptone 2
    Yeast Extract 0.2
    Glucose 0.2
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
      Suspend 7.89 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Agar/5

    $31.01$559.94
    SKU Pack Size Price
    SMA50103 6 x 1kg $559.94
    SMA50102 1000g $103.78
    SMA50101 250g $31.01
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    Formula
    g/l
    Peptone 2
    Yeast Extract 0.2
    Glucose 2
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
    Agar 17
      Suspend 24.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • HL5 Medium without Glucose

    $27.37$584.89
    SKU Pack Size Price
    HLB0101 250g $27.37
    HLB0103 6 x 1kg $584.89
    HLB0102 1000g $103.78
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    KH2PO4 0.5
    Na2HPO4 0.5
      Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLG0101 250g $25.47
    HLG0103 6 x 1kg $461.71
    HLG0102 1000g $83.68
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    Glucose 13.5
    KH2PO4 0.5
    Na2HPO4 0.5
      Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLC0101 250g $25.47
    HLC0103 6 x 1kg $451.84
    HLC0102 1000g $83.68
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    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    Glucose 10
      Suspend 26.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLD0102 1000g $100.14
    HLD0103 6 x 1kg $552.32
    HLD0101 250g $27.37
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    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
      Suspend 16.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLE2 1000g $87.32
    HLE3 6 x 1kg $471.59
    HLE1 250g $26.33
    HL5 supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    Glucose 13.5
    KH2PO4 0.5
    Na2HPO4 0.5
    FM Vitamins and Micro-elements 0.01
      Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLF1 250g $28.24
    HLF2 1000g $105.51
    HLF3 6 x 1kg $569.82
    HL5 Medium supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dictyostelium Discoideum who require additional nutritional elements for starting up vigorous growth or protein synthesis.
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    KH2PO4 0.5
    Na2HPO4 0.5
    FM Vitamins and Micro-elements 0.01
      Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLH1 250g $26.33
    HLH3 6 x 1kg $461.71
    HLH2 1kg $85.59
    HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.
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    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    Glucose 10
    FM Vitamins and Micro-elements 0.01
      Suspend 26.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLI2 1kg $101.87
    HLI1 250g $28.24
    HLI3 6 x 1kg $562.37
    HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.
    Select options
    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    FM Vitamins and Micro-elements 0.01
      Suspend 16.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • FM Minimal Medium

    $51.98$935.55
    SKU Pack Size Price
    FMM0102 1kg $173.25
    FMM0103 6 x 1kg $935.55
    FMM0101 250g $51.98
    Select options
    Formula
    mg/l
    Amino Acids
    Arg 700
    Asp 300
    Cys 200
    GluA 500
    Gly 900
    His 300
    Ile 600
    Leu 900
    Lys 900
    Met 300
    Phe 500
    Pro 800
    Thr 500
    Trp 200
    Val 700
    Vitamins
    Biotin 0.02
    Cyanocobalamin 0.01
    Folic Acid 0.2
    Lipoic Acid 0.4
    Riboflavin 0.5
    Thiamine 0.6
    Micro Elements
    Na2EDTA.2H2O 4.84
    ZnSO4 2.3
    H3BO3 1.11
    MnCl2.4H2O 0.51
    CoCl2.6H2O 0.17
    CuSO4.5H2O 0.15
    (NH4)6Mo7O24.4H2O 0.1
    Minerals
    NH4Cl 53.5
    CaCl2.2H2O 2.94
    FeCl3 16.2
    MgCl2.6H2O 81.32
    KH2PO4 870
    Carbon Source
    Glucose 10000
    Total 19334.87
      Suspend 19.3 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0202 1kg $173.25
    FMM0203 6 x 1kg $935.55
    FMM0201 250g $51.98
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    Suspend 19.3 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0502 1kg $173.25
    FMM0503 6 x 1kg $935.55
    FMM0501 250g $51.98
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    Suspend 11.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0602 1kg $173.25
    FMM0603 6 x 1kg $935.55
    FMM0601 250g $51.98
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    Suspend 11.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0302 1kg $173.25
    FMM0303 6 x 1kg $935.55
    FMM0301 250g $51.98
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    Suspend 19.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0402 1kg $173.25
    FMM0403 6 x 1kg $935.55
    FMM0401 250g $51.98
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    Suspend 17.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0702 1000g $173.25
    FMM0703 6 x 1kg $935.55
    FMM0701 250g $51.98
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    Suspend 18.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMM0802 1kg $173.25
    FMM0803 6 x 1kg $935.55
    FMM0801 250g $51.98
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    Suspend 18.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    FMA03 250g $103.95
    FMA02 100g $51.98
    FM Amino acid drop-out mixture is based on the Amino acids present in FM Minimal medium. These Amino acids form a basis for many "Drop-Out" mixtures to select for auxotrophic requirements and transformants. Each FM Drop-Out mixture contain all components except for one or two essential Amino acids, i.e. the "dropped out" supplements. FM Amino acids drop-out mixtures are used in combination with FM Minimal medium w/o Amino acids or FM Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium. Complete Supplement Mixture formulations are available in 25 gram and 250 gram pack sizes. Please enquire about custom made formulations.
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    Formula
    mg/l
    Amino Acids
    Arg 700
    Asp 300
    Cys 200
    GluA 500
    Gly 900
    His 300
    Ile 600
    Leu 900
    Lys 900
    Met 300
    Phe 500
    Pro 800
    Thr 500
    Trp 200
    Val 700
    Total 8300 mg/l
     
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  • SKU Pack Size Price
    FMA47 100g $51.98
    FMA48 250g $103.95
  • SKU Pack Size Price
    FMA06 250g $103.95
    FMA05 100g $51.98
  • SKU Pack Size Price
    FMA09 250g $103.95
    FMA08 100g $51.98
  • SKU Pack Size Price
    FMA12 250g $103.95
    FMA11 100g $51.98
  • SKU Pack Size Price
    FMA15 250g $103.95
    FMA14 100g $51.98
  • SKU Pack Size Price
    FMA18 250g $103.95
    FMA17 100g $51.98
  • SKU Pack Size Price
    FMA21 250g $103.95
    FMA20 100g $51.98
  • SKU Pack Size Price
    FMA24 250g $103.95
    FMA23 100g $51.98
  • SKU Pack Size Price
    FMA27 250g $103.95
    FMA26 100g $51.98
  • SKU Pack Size Price
    FMA30 250g $103.95
    FMA29 100g $51.98
  • SKU Pack Size Price
    FMA33 250g $103.95
    FMA32 100g $51.98
  • SKU Pack Size Price
    FMA36 250g $103.95
    FMA35 100g $51.98
  • SKU Pack Size Price
    FMA39 250g $103.95
    FMA38 100g $51.98
  • SKU Pack Size Price
    FMA42 250g $103.95
    FMA41 100g $51.98
  • SKU Pack Size Price
    FMA45 250g $103.95
    FMA44 100g $51.98
  • SIH Medium

    $51.98$935.55
    SKU Pack Size Price
    SIH0102 1kg $173.25
    SIH0103 6 x 1kg $935.55
    SIH0101 250g $51.98
    SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1.1 mM) and increased levels of Lysine (8.5 mM) and Tryptophane (1.7 mM). Concentrations of Cysteine, Glutamic acid, Methionine, Phenylalanine, Threonine are slightly increased as well in SIH. The alterations in amino acid formulation of SIH medium resulted in a more even and better amino acid utilisation. Cell density of D. discoideum rose up to levels in excess of 5 x 107 cells compared to FM medium with cell density levels of 3 x 107. Improvement of a synthetic medium for Dictyostelium discoideum, Sang-In Han, Karl Friehs and Erwin Flaschel, Process Biochemistry, 39 (8), 925 � 930, 2004. Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor. Sang-In Han, Karl Friehs and Erwin Flaschel, Process Biochemistry, 39, 585 - 589, 2004.
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    Formula
    mg/l
    Amino Acids
    Arg 700
    Asp 300
    Asp A 150
    Cys 300
    GluA 545
    Gly 900
    His 300
    Ile 600
    Leu 900
    Lys 1250
    Met 350
    Phe 550
    Pro 800
    Thr 500
    Trp 350
    Val 700
    Vitamins
    Biotin 0.02
    Cyanocobalamin 0.01
    Folic Acid 0.2
    Lipoic Acid 0.4
    Riboflavin 0.5
    Thiamine 0.6
    Micro Elements
    Na2EDTA.2H2O 4.84
    ZnSO4 2.3
    H3BO3 1.11
    MnCl2.4H2O 0.51
    CoCl2.6H2O 0.17
    CuSO4.5H2O 0.15
    (NH4)6Mo7O24.4H2O 0.1
    Minerals
    NH4Cl 53.5
    CaCl2.2H2O 2.94
    FeCl3 16.2
    MgCl2.6H2O 81.32
    KH2PO4 870
    Carbon Source
    Glucose 10000
    Total 20326
      Suspend 20.3 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SIH medium w/o NH4Cl

    $51.98$935.55
    SKU Pack Size Price
    SIH0502 1kg $173.25
    SIH0503 6 x 1kg $935.55
    SIH0501 250g $51.98
    Select options
    Suspend 20.3 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SIH medium w/o Amino acids

    $51.98$935.55
    SKU Pack Size Price
    SIH0602 1kg $173.25
    SIH0603 6 x 1kg $935.55
    SIH0601 250g $51.98
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    Suspend 11.1 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    SIH0702 1kg $173.25
    SIH0703 6 x 1kg $935.55
    SIH0701 250g $51.98
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    Suspend 11.1 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SIH medium w/o Methionine

    $51.98$935.55
    SKU Pack Size Price
    SIM0102 1kg $173.25
    SIM0103 6 x 1kg $935.55
    SIM0101 250g $51.98
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    Suspend 20.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    SIH1002 1kg $173.25
    SIH1003 6 x 1kg $935.55
    SIH1001 250g $51.98
    Select options
    Suspend 18.4 gram powdered medium in 1 litre distilled water Store dry at room temperature
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