Yeast Nitrogen Base w/o Amino acids LoFlo is a low fluorescence YNB type medium.
By omitting Folic acid and Riboflavin from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium.
Yeast Nitrogen Base w/o Amino acids LoFlo is excellent for whole-cell fluorescence experiments and because of its low fluorescence background the fluorescence of cells can be measured directly in the medium.
Yeast Nitrogen Base w/o Amino acids LoFlo is well suited for intracellular pH measurements in cells expressing pHluorin in combination with Raman spectroscopy.
Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature