SIH Medium

SKU Pack Size Price
SIH0101250g $54.58
SIH01021kg $181.91
SIH01036 x 1kg $982.33

SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities.

SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977).

The main difference between SIH and FM is the novel addition of Aspartic acid (1.1 mM) and increased levels of Lysine (8.5 mM) and Tryptophane (1.7 mM). Concentrations of Cysteine, Glutamic acid, Methionine, Phenylalanine, Threonine are slightly increased as well in SIH.

The alterations in amino acid formulation of SIH medium resulted in a more even and better amino acid utilisation. Cell density of D. discoideum rose up to levels in excess of 5 x 107 cells compared to FM medium with cell density levels of 3 x 107.

Improvement of a synthetic medium for Dictyostelium discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 � 930, 2004.

Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor.
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39, 585 – 589, 2004.

From: $54.58

From: $54.58
SKU: SIH01CFG Category:

Description

Formula
mg/l
Amino Acids
Arg700
Asp300
Asp A150
Cys300
GluA545
Gly900
His300
Ile600
Leu900
Lys1250
Met350
Phe550
Pro800
Thr500
Trp350
Val700
Vitamins
Biotin0.02
Cyanocobalamin0.01
Folic Acid0.2
Lipoic Acid0.4
Riboflavin0.5
Thiamine0.6
Micro Elements
Na2EDTA.2H2O4.84
ZnSO42.3
H3BO31.11
MnCl2.4H2O0.51
CoCl2.6H2O0.17
CuSO4.5H2O0.15
(NH4)6Mo7O24.4H2O0.1
Minerals
NH4Cl53.5
CaCl2.2H2O2.94
FeCl316.2
MgCl2.6H2O81.32
KH2PO4870
Carbon Source
Glucose10000
Total20326

 

Suspend 20.3 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

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