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LB-Broth Low Salt, Lennox L Broth
LB Broth Lennox is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize, support a luxurious growth of E. coli cells.
Sodium ions for transport and osmotic balance are provided by Sodium chloride. The concentration of NaCl in LB Lennox is half the concentration of the LB Miller Broth formulation and ten times the concentration of LB Luria Broth formulation. These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain.
Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955).
Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).
Documents
Title
Type
Link
Description
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Formula
g/l
Tryptone
10
Yeast extract
5
NaCl
5
Final pH
7.0 ± 0.2 at 25°C
Suspend 20 gram powdered medium in 1 litre distilled water
Store dry at room temperature
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Orders within the UK
Orders within the UK with an order value of £250 or more (excluding VAT) will be delivered free of charge. Orders within the UK with an order value of less than £250 (excluding VAT) will be charged delivery of £12.50.
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Orders from the US
Deliveries to the US start from less than $32. Formedium can assist you with or organise your US import permits. All freight charges are based on weight of order. Import tax is the responsibility of the purchaser and their clearing agents.
Orders from the rest of the world
Formedium deliver worldwide. Customs restrictions may apply so please do contact us if you have any queries. The cost of shipping orders will vary by destination and weight. All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice. Import tax is the responsibility of the purchaser and their clearing agents.
Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications. If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or sales@formedium.com.
We are pleased to offer unpublished discounts on large volume purchases, please email us at info@formedium.com with your enquiry or complete the enquiry form below.
To review our research publications library where Formedium products have been used, please view the research publications page.
Name & Registered Office: Formedium Ltd. Norfolk House, 22-24 Market Place, Swaffham, Norfolk, England, PE37 7QH Registered in England and Wales. Company No. 04351225. VAT No. GB 803727440
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