C22H24N2O8·HCl·0.5H2O·0.5C2H6O = 512.94
Doxycycline is a bacteriostatical Tetracycline like antibiotic. The antibiotic is effective against both gram-positive and gram-negative bacteria. It binds reversible to the ribosomal 30 S unit, preventing binding of aminoacyl transfer RNA and hence inhibiting protein synthesis.
Doxycycline is an effective repressor of the TetO7 promoter system.
Media containing 10 micro-gram Doxycycline effectively repress the TetO7 promoter.