Yeast Nitrogen Base without Amino Acids

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Yeast Nitrogen Base without Amino Acids

Saccharomyces Cerevisiae Yeast Nitrogen Base without Amino Acids

Yeast Nitrogen Base media have been prepared according the to formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB).  YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.

Besides the original formulation of YNB, Formedium™ produces many variations of this medium without one or more components.  These YNB without ‘X’ media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component.

* Please note all formulations within this product range are based upon Yeast Nitrogen Base without Amino Acids and without the Component/Components selected.

View technical data.

  • SKU Pack Size Price
    CYN0401100g £20.07
    CYN0402250g £43.66
    CYN0405500g £88.87
    CYN04101000g £162.40
    Yeast Nitrogen Base without amino acids (YNB w/o AA) is used for selecting yeasts based on amino acid and carbohydrate requirements. In Yeast Nitrogen Base with out amino acids Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells.Compared to full Yeast Nitrogen Base medium, Yeast Nitrogen Base without amino acids lacks Histidine, Methionine and Trypthophan. In combination with Drop Out medium supplement mixtures  Yeast Nitrogen Base without Amino acids provides an excellent medium to cultivate and to select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins.The addition of a carbon source like glucose, galactose or raffinose is required. Synthetic Dextrose Minimal medium (SD medium) and Synthetic Complete medium (SC medium) are based upon Yeast Nitrogen Base w/o amino acids and are complemented with glucose, galactose and raffinose as carbon sources.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4101100g £20.51
    CYN4102500g £90.63
    CYN41101000g £166.48
    Para-Amino benzoic acid is omitted from this formulation of Yeast Nitrogen base w/o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of para-Amino Benzoic acid or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3101100g £20.51
    CYN3102500g £90.63
    CYN31101000g £166.48
    Biotin is omitted from this formulation of Yeast Nitrogen base w/o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of biotin or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled waterStore dry at room temperature
  • SKU Pack Size Price
    CYN1501100g £20.51
    CYN1502500g £90.63
    CYN15101000g £166.48
    Boric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2501100g £20.51
    CYN2502500g £90.63
    CYN25101000g £166.48
    Calcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element.Suspend 6.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3301100g £20.51
    CYN3302500g £90.63
    CYN33101000g £166.48
    Calcium-pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium-pantothenate or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled waterStore dry at room temperature
  • SKU Pack Size Price
    CYN0901100g £20.51
    CYN0902250g £41.61
    CYN0905500g £90.63
    CYN09101000g £166.48
    Cupric sulphate, CuSO4.5H2O, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper or to replace this element.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1301100g £20.51
    CYN1302500g £90.63
    CYN13101000g £166.48
    Cupric sulphate, CuSO4.5H2O, and Ferric chloride, FeCl3 are omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper and iron or to replace these elements.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3501100g £20.51
    CYN3502500g £90.63
    CYN35101000g £166.48
    Folic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Folic acid or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN6501100g £20.51
    CYN6502250g £43.66
    CYN6505500g £88.87
    CYN65101kg £162.40
    Yeast Nitrogen Base w/o Amino acids LoFlo is a low fluorescence YNB type medium.By omitting Folic acid and Riboflavin from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium.Yeast Nitrogen Base w/o Amino acids LoFlo is excellent for  whole-cell fluorescence experiments and because of its low fluorescence background the fluorescence of cells can be measured directly in the medium.Yeast Nitrogen Base w/o Amino acids LoFlo is well suited for intracellular pH measurements in cells expressing pHluorin in combination with Raman spectroscopy.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3701100g £20.51
    CYN3702500g £90.63
    CYN37101000g £166.48
    Inositol is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of inositol or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4901100g £20.51
    CYN4902500g £90.63
    CYN49101000g £166.48
    Potassium Iodide, KI, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iodine or to replace this element.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1101100g £20.51
    CYN1102500g £90.63
    CYN11101000g £166.48
    Ferric chloride, FeCl3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iron or to replace this element.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2701100g £20.51
    CYN2702500g £90.63
    CYN27101000g £166.48
    Magnesium sulphate, MgSO4.7H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of magnesium or to replace this element.Suspend 6.2 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1901100g £20.51
    CYN1902500g £90.63
    CYN19101000g £166.48
    Manganese sulphate, MnSO4.H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Manganese or to replace this element.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2101100g £20.51
    CYN2102500g £90.63
    CYN21101000g £166.48
    Molybdic acid Sodium salt, MoNa2O5.2H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Molybdene or to replace this element.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3901100g £20.51
    CYN3902500g £90.63
    CYN39101000g £166.48
    Nicotinic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN0801100g £22.94
    CYN0802250g £52.15
    CYN0803500g £94.82
    CYN08041000g £172.55
    The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines.Suspend 5.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN6701100g £22.94
    CYN6702250g £52.15
    CYN6703500g £94.82
    CYN67041kg £172.55
    The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines.By omitting Potassium DiHydrogen Phosphate (KH2PO4), not only Phosphate (PO4-3) but also Potassium (K ) is omitted.As a result yeasts are not only limited in Phosphate, but also in Potassium. This might cause problems in osmo-molarity and intercellular Potassium ion concentrations finally resulting in poor cell growth and low yields.To avoid this problem an extra 550 mg/l (7.35 mM) of KCl is added. By adding this concentration of KCl the original concentration of K as present in YNB is restored. The extra addition of Chlorine is in almost all cases no problems to your cells.Suspend 6.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4301100g £20.51
    CYN4302500g £90.63
    CYN43101000g £166.48
    Pyridoxine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of pyridoxine or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4501100g £20.51
    CYN4502500g £90.63
    CYN45101000g £166.48
    Riboflavin is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2901100g £20.51
    CYN2902500g £90.63
    CYN29101000g £166.48
    Sodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element.Suspend 6.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4701100g £20.51
    CYN4702500g £90.63
    CYN47101000g £166.48
    Thiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2301100g £20.51
    CYN2302500g £90.63
    CYN23101000g £166.48
    Zinc sulphate, ZnSO4.7H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Zinc or to replace this element.Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature