You are here:>>>Yeast Nitrogen Base without Amino Acids

Yeast Nitrogen Base without Amino Acids

Saccharomyces Cerevisiae Yeast Nitrogen Base without Amino Acids

Yeast Nitrogen Base media have been prepared according the to formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB).  YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.

Besides the original formulation of YNB, Formedium™ produces many variations of this medium without one or more components.  These YNB without ‘X’ media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component.

* Please note all formulations within this product range are based upon Yeast Nitrogen Base without Amino Acids and without the Component/Components selected.

View technical data.

  • SKU Pack Size Price
    CYN0402 250g $68.61
    CYN0405 500g $139.64
    CYN0410 1000g $255.20
    CYN0401 100g $31.53
    Yeast Nitrogen Base without amino acids (YNB w/o AA) is used for selecting yeasts based on amino acid and carbohydrate requirements. In Yeast Nitrogen Base with out amino acids Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full Yeast Nitrogen Base medium, Yeast Nitrogen Base without amino acids lacks Histidine, Methionine and Trypthophan. In combination with Drop Out medium supplement mixtures  Yeast Nitrogen Base without Amino acids provides an excellent medium to cultivate and to select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins. The addition of a carbon source like glucose, galactose or raffinose is required. Synthetic Dextrose Minimal medium (SD medium) and Synthetic Complete medium (SC medium) are based upon Yeast Nitrogen Base w/o amino acids and are complemented with glucose, galactose and raffinose as carbon sources. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4102 500g $142.41
    CYN4110 1000g $261.61
    CYN4101 100g $32.22
    Para-Amino benzoic acid is omitted from this formulation of Yeast Nitrogen base w/o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of para-Amino Benzoic acid or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3102 500g $142.41
    CYN3110 1000g $261.61
    CYN3101 100g $32.22
    Biotin is omitted from this formulation of Yeast Nitrogen base w/o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of biotin or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1502 500g $142.41
    CYN1510 1000g $261.61
    CYN1501 100g $32.22
    Boric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2502 500g $142.41
    CYN2510 1000g $261.61
    CYN2501 100g $32.22
    Calcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element. For technical data please click here. Suspend 6.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3302 500g $142.41
    CYN3310 1000g $261.61
    CYN3301 100g $32.22
    Calcium-pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium-pantothenate or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN0902 250g $65.38
    CYN0905 500g $142.41
    CYN0910 1000g $261.61
    CYN0901 100g $32.22
    Cupric sulphate, CuSO4.5H2O, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper or to replace this element. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1302 500g $142.41
    CYN1310 1000g $261.61
    CYN1301 100g $32.22
    Cupric sulphate, CuSO4.5H2O, and Ferric chloride, FeCl3 are omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper and iron or to replace these elements. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3502 500g $142.41
    CYN3510 1000g $261.61
    CYN3501 100g $32.22
    Folic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Folic acid or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN6502 250g $68.61
    CYN6505 500g $139.64
    CYN6510 1kg $255.20
    CYN6501 100g $32.22
    Yeast Nitrogen Base w/o Amino acids LoFlo is a low fluorescence YNB type medium. By omitting Folic acid and Riboflavin from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium. Yeast Nitrogen Base w/o Amino acids LoFlo is excellent for  whole-cell fluorescence experiments and because of its low fluorescence background the fluorescence of cells can be measured directly in the medium. Yeast Nitrogen Base w/o Amino acids LoFlo is well suited for intracellular pH measurements in cells expressing pHluorin in combination with Raman spectroscopy. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3702 500g $142.41
    CYN3710 1000g $261.61
    CYN3701 100g $32.22
    Inositol is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of inositol or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4902 500g $142.41
    CYN4910 1000g $261.61
    CYN4901 100g $32.22
    Potassium Iodide, KI, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iodine or to replace this element. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1102 500g $142.41
    CYN1110 1000g $261.61
    CYN1101 100g $32.22
    Ferric chloride, FeCl3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iron or to replace this element. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2702 500g $142.41
    CYN2710 1000g $261.61
    CYN2701 100g $32.22
    Magnesium sulphate, MgSO4.7H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of magnesium or to replace this element. For technical data please click here. Suspend 6.2 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1902 500g $142.41
    CYN1910 1000g $261.61
    CYN1901 100g $32.22
    Manganese sulphate, MnSO4.H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Manganese or to replace this element. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2102 500g $142.41
    CYN2110 1000g $261.61
    CYN2101 100g $32.22
    Molybdic acid Sodium salt, MoNa2O5.2H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Molybdene or to replace this element. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3902 500g $142.41
    CYN3910 1000g $261.61
    CYN3901 100g $32.22
    Nicotinic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN0802 250g $81.95
    CYN0803 500g $149.00
    CYN0804 1000g $271.14
    CYN0801 100g $36.04
    The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. For technical data please click here. Suspend 5.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN6702 250g $81.95
    CYN6703 500g $149.00
    CYN6704 1kg $271.14
    CYN6701 100g $36.04
    The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. By omitting Potassium DiHydrogen Phosphate (KH2PO4), not only Phosphate (PO4-3) but also Potassium (K ) is omitted. As a result yeasts are not only limited in Phosphate, but also in Potassium. This might cause problems in osmo-molarity and intercellular Potassium ion concentrations finally resulting in poor cell growth and low yields. To avoid this problem an extra 550 mg/l (7.35 mM) of KCl is added. By adding this concentration of KCl the original concentration of K as present in YNB is restored. The extra addition of Chlorine is in almost all cases no problems to your cells. Suspend 6.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4302 500g $142.41
    CYN4310 1000g $261.61
    CYN4301 100g $32.22
    Pyridoxine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of pyridoxine or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4502 500g $142.41
    CYN4510 1000g $261.61
    CYN4501 100g $32.22
    Riboflavin is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2902 500g $142.41
    CYN2910 1000g $261.61
    CYN2901 100g $32.22
    Sodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element. For technical data please click here. Suspend 6.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4702 500g $142.41
    CYN4710 1000g $261.61
    CYN4701 100g $32.22
    Thiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2302 500g $142.41
    CYN2310 1000g $261.61
    CYN2301 100g $32.22
    Zinc sulphate, ZnSO4.7H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Zinc or to replace this element. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature