Yeast Nitrogen Base without Amino Acids and without Ammonium Sulphate

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Yeast Nitrogen Base without Amino Acids and without Ammonium Sulphate

Saccharomyces Cerevisiae Yeast Nitrogen Base without Amino Acids and without Ammonium Sulphate

Yeast Nitrogen Base media have been prepared according to the formulae of Wickerham (1943-51) and Burkholder (1943).  Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB).  YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.

Besides the original formulation of YNB, Formedium™ produces many variations of this medium without one or more components.  These YNB without ‘X’ media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component.

* Please note all formulations within this product range are based upon Yeast Nitrogen Base without Amino Acids, without Ammonium Sulphate and without the Component/Components selected.

View technical data.

  • SKU Pack Size Price
    CYN10001100g £20.51
    CYN10005500g £90.63
    CYN100101000g £166.48
    In Translucent K free medium ammonium phosphate (0.92 g/l) substitutes for potassium phosphate to reduce the amount of potassium to a minimum. Both Folic acid and Riboflavin are omitted from the medium to make it suitable for  fluorescence measurements with yeast cultures.For technical data please click here.Suspend 1.75 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN7501100g £20.51
    CYN7505500g £90.63
    CYN75101000g £166.48
    In Translucent K free medium ammonium phosphate (0.92 g/l) substitutes for potassium phosphate to reduce the amount of potassium to a minimum.Suspend 1.75 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN0501100g £27.68
    CYN0502250g £60.09
    CYN0505500g £109.48
    CYN05101000g £199.23
    Yeast Nitrogen Base without Amino Acids and without Ammonium Sulfate has the same formulation as Yeast Nitrogen Base without Amino Acids except that Ammonium Sulfate as a source of Nitrogen has been omitted to test the ability of yeast cells to assimilate different nitrogen sources for example Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4201100g £28.23
    CYN4202500g £111.58
    CYN42101000g £203.31
    Para-Amino benzoic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of para-Amino Benzoic acid or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3201100g £28.23
    CYN3202500g £111.58
    CYN32101000g £203.31
    Biotin is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of biotin or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1601100g £28.23
    CYN1602500g £111.58
    CYN16101000g £203.31
    Boric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2601100g £28.23
    CYN2602500g £111.58
    CYN26101000g £203.31
    Calcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3401100g £28.23
    CYN3402500g £111.58
    CYN34101000g £203.31
    Calcium-pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium-pantothenate or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1001100g £28.23
    CYN1002500g £111.58
    CYN10101000g £203.31
    Cupric sulphate, CuSO4.5H2O, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1701100g £28.23
    CYN1702500g £111.58
    CYN17101000g £203.31
    Cupric sulphate, CuSO4.5H2O, and Ferric chloride, FeCl3 are omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper and iron or to replace these elements.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3601100g £28.23
    CYN3602500g £111.58
    CYN36101000g £203.31
    Folic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Folic acid or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN6201100g £27.68
    CYN6202250g £60.09
    CYN6205500g £112.46
    CYN62101000g £199.23
    Yeast Nitrogen Base w/o Amino acids w/o Ammonium sulphate LoFlo is a low fluorescence YNB type medium.By omitting Folic acid and Riboflavine from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium.Yeast Nitrogen Base w/o Amino acids w/o Ammonium sulphate LoFlo is excellent for  whole-cell fluorescence experiments and because of its low fluorescence background the fluorescence of cells can be measured directly in the medium.Yeast Nitrogen Base w/o Amino acids w/o Ammonium sulphate LoFlo is well suited for intracellular pH measurements in cells expressing pHluorin in combination with Raman spectroscopy.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3801100g £28.23
    CYN3802500g £111.58
    CYN38101000g £203.31
    Inositol is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of inositol or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1801100g £28.23
    CYN1802500g £111.58
    CYN18101000g £203.31
    Potassium Iodide, KI, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iodine or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN1201100g £28.23
    CYN1202500g £111.58
    CYN12101000g £203.31
    Ferric chloride, FeCl3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iron or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2801100g £28.23
    CYN2802500g £111.58
    CYN28101000g £203.31
    Magnesium sulphate, MgSO4.7H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of magnesium or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.2 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2001100g £28.23
    CYN2002500g £111.58
    CYN20101000g £203.31
    Manganese sulphate, MnSO4.H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Manganese or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2201100g £28.23
    CYN2202500g £111.58
    CYN22101000g £203.31
    Molybdic acid Sodium salt, MoNa2O5.2H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Molybdene or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4001100g £28.23
    CYN4002500g £111.58
    CYN40101000g £203.31
    Nicotinic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN6801100g £22.94
    CYN6802250g £52.15
    CYN6803500g £94.82
    CYN68041kg £172.55
    The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines.By omitting Potassium DiHydrogen Phosphate (KH2PO4), not only Phosphate (PO4-3) but also Potassium (K ) is omitted.As a result yeasts are not only limited in Phosphate, but also in Potassium. This might cause problems in osmo-molarity and intercellular Potassium ion concentrations finally resulting in poor cell growth and low yields.To avoid this problem an extra 550 mg/l (7.35 mM) of KCl is added. By adding this concentration of KCl the original concentration of K as present in YNB is restored. The extra addition of Chlorine is in almost all cases no problems to your cells.Suspend 1.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4401100g £28.23
    CYN4402500g £111.58
    CYN44101000g £203.31
    Pyridoxine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of pyridoxine or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4601100g £28.23
    CYN4602500g £111.58
    CYN46101000g £203.31
    Riboflavin is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN3001100g £28.23
    CYN3002500g £111.58
    CYN30101000g £203.31
    Sodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.8 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN4801100g £28.23
    CYN4802500g £111.58
    CYN48101000g £203.31
    Thiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN2401100g £28.23
    CYN2402500g £111.58
    CYN24101000g £203.31
    Zinc sulphate, ZnSO4.7H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Zinc or to replace this element.Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.Suspend 1.9 gram powdered medium in 1 litre distilled water Store dry at room temperature