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Media for Optimal Cell Growth and Yield of
Escherichia Coli Cultures

LB Media (Luria-Bertani) are common bacterial growth media for Escherichia Coli.

Although already described in the fifties in the early days of phage genetics these media are still widely used in molecular biology.

The two main components of LB media are Tryptone and Yeast Extract.  Tryptone is used in a concentration of 10 gram / litre and Yeast Extract in a concentration of 5 gram / litre.  Many variations of LB medium only differs in the concentration of NaCl.  All LB-Media are listed in order of increasing concentration of NaCl.

LB Medium Miller is the High Salt LB medium type with 10 gram /litre whereas LB Medium Lennox is the Low Salt LB medium type with 5 gram / litre.  L-Broth only contain 0.5 gram of NaCl per litre and is used mainly when working with phage P1 where CaCl2 is added for efficient adherence of the phage to the cell.  In LBM media Mg2+ is added to enhance adsorption of phage lambda to the cells.  Tryptone and Yeast Extract do not contain enough Mg2+ for optimal adsorption.

Many synonymous names are know for several different type of LB media.  We have tried to rank them all.

In case a medium cannot be found please also look at the synonymous names.

Tryptone broth is a moderately rich medium for growth and cultivation of Escherichia Coli.

Terrific Broth is a rich medium compared to LB and Tryptone Media. The medium is developed for higher density growth of Escherichia Coli cells and higher yield of plasmid DNA compared to LB and Tryptone broth.

Super Broth is an even richer medium developed for obtaining high yields of lambda bacteriophage in liquid lysates, Botstein, D. et al.

Powdered Media Storage and Preparation

Ready made powdered media are hygroscopic and must be protected from atmospheric moisture.  Always reseal tightly after opening.

Store the medium dry at room temperature.

Preparing media in a concentrated form is not recommended.  Some salt and protein complexes may precipitate in a concentrated solution.

Sterilise the medium in a validated autoclave at 1 kg/cm2 (15 psi) at 121°C. for 15 minutes.  Higher temperatures or exceeding the period of time for autoclavation may cause a  brown decolourisation of the medium due to caramelisation of the glucose present in the medium.  Denaturising of proteins may occur as well.  Both will result in poor cell growth.

References:
Botstein, D. et al, Mol. Biol., 91, 439, (1975)
Lennox, E.S., Virology, 1, 190, (1955).
Luria, S.E. and Burrous, J.W., J. Bacteriol. 74, 471, (1957).
Luria, S.E. et al., Virology, 12, 348, (1960).
Blattner, F., et al. Science, 196, 161, (1977).
Miller, J.H., Experiments in Molecular Genetics, C.S.H.   Press, N.Y., (1972)

  • LB-Broth w/o NaCl

    £13.69£281.71
    SKU Pack Size Price
    LBO0101250g £13.69
    LBO01021000g £51.09
    LBO01036 x 1kg £281.71
  • Sabouraud Agar

    £11.08£32.73
    SKU Pack Size Price
    SAB0102250g £11.08
    SAB0105500g £18.70
    SAB01101kg £32.73
  • LB Agar w/o NaCl

    £15.48£331.01
    SKU Pack Size Price
    LBO0201250g £15.48
    LBO02021000g £60.00
    LBO02036 x 1kg £331.01
  • L-Broth

    £14.59£204.24
    SKU Pack Size Price
    LBL0101250g £14.59
    LBL01021000g £37.40
    LBL01036 x 1kg £204.24
    Luria Broth Base (Miller�s Modification)
    Luria Broth Base or L-Broth is based upon the Luria Broth formulation as originally developed by Miller for cultivation and maintenance of E. coli cells in molecular biology.L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize, support a luxurious growth of E. coli cells.Sodium ions for transport and osmotic balance are provided by Sodium chloride.The concentration of NaCl in L-Broth is low compared to both LB Miller and LB Lennox formulations, respectively 10% and 5% of the NaCl concentration is present in both formulations.These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain.Miller, J.H., Experiments in molecular genetics, Cold Spring harbour Laboratory, Cold Spring harbour, New York, (1972). Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955). Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
  • L-Broth Agar

    £15.48£331.01
    SKU Pack Size Price
    LBL0201250g £15.48
    LBL02021000g £60.33
    LBL02036 x 1kg £331.01
    Luria Agar Base (Miller's Modification)
  • L-Broth Top Agar

    £17.92£61.22
    SKU Pack Size Price
    LBL0301250g £17.92
    LBL03021000g £61.22
  • VLB-Agar Lennox

    £2.79£200.12
    SKU Pack Size Price
    VLBAL1L1 Litre pack £2.79
    VLBAL2L2 Litre pack £4.23
    VLBAL5L5 Litre pack £8.24
    VLBAL10L10L pack £14.03
    VLBAL20L20 Litre pack £26.27
    VLBAL0250250g £10.91
    VLBAL0500500g £21.60
    VLBAL10001000g £37.18
    VLBAL60006 x 1kg £200.12
  • VLB-Broth Lennox

    £2.56£167.40
    SKU Pack Size Price
    VLBBL1L1 Litre pack £2.56
    VLBBL2L-12 Litre pack £4.12
    VLBBL5L5 Litre pack £5.90
    VLBBL10L10L pack £10.24
    VLBBL20L20 Litre pack £16.37
    VLBBL0250250g £10.80
    VLBBL0500500g £19.59
    VLBBL10001kg £35.73
    VLBBL60006 x 1kg £167.40
  • LB-Broth Lennox

    £2.56£167.40
    SKU Pack Size Price
    LBX1L1 Litre pack £2.56
    LBX2L2 Litre pack £4.12
    LBX5L5 Litre pack £5.90
    LBX10L10L pack £10.24
    LBX20L20 Litre pack £16.37
    LBX0101250g £10.80
    LBX0104500g £19.59
    LBX01021kg £35.73
    LBX01036 x 1kg £167.40
    LB-Broth Low Salt, Lennox L Broth
    LB Broth Lennox is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize,  support a luxurious growth of E. coli cells.Sodium ions for transport and osmotic balance are provided by Sodium chloride. The concentration of NaCl in LB Lennox is half the concentration of the LB Miller Broth formulation and ten times the concentration of LB Luria Broth formulation. These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain.Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955). Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).
  • VLB-Agar Miller

    £3.23£265.57
    SKU Pack Size Price
    VLBAM1L1 Litre pack £3.23
    VLBAM2L2 Litre pack £4.90
    VLBAM5L5 Litre pack £11.47
    VLBAM10L10L pack £21.04
    VLBAM20L20 Litre pack £39.74
    VLBAM0250250g £15.48
    VLBAM0500500g £28.28
    VLBAM10001000g £49.09
    VLBAM60006 x 1kg £265.57
  • VLB-Broth Miller

    £2.56£185.88
    SKU Pack Size Price
    VLBBM1L1 Litre pack - 25g £2.56
    VLBBM2L2 Litre pack - 50g £3.57
    VLBBM5L5 Litre pack - 125 gram £7.02
    VLBBM10L10 Litre pack - 250g £11.91
    VLBBM20L20 Litre pack - 500g £22.26
    VLBBM0250250g £11.91
    VLBBM0500500g £22.26
    VLBBM10001000g £36.96
    VLBBM15001500g £52.65
    VLBBM60006 x 1kg £185.88
     
  • LB-Agar Lennox

    £2.79£200.12
    SKU Pack Size Price
    LBXA1L1 Litre pack £2.79
    LBXA2L2 Litre pack £4.23
    LBXA5L5 Litre pack £8.24
    LBX0201250g £10.91
    LBXA10L10L pack £14.03
    LBXA20L20 Litre pack £26.27
    LBX02021000g £37.18
    LBX02036 x 1kg £200.12
    LB-Agar Low Salt, Lennox L Agar
  • LB-Top Agar Lennox

    £10.58£194.22
    SKU Pack Size Price
    LBX0301250g £10.58
    LBX03021000g £35.95
    LBX03036 x 1kg £194.22
    LB-Top Agar Low Salt, Lennox L
  • LBM-Broth Lennox

    £10.36£162.50
    SKU Pack Size Price
    LXM0101250g £10.36
    LXM01021000g £34.73
    LXM01036 x 1kg £162.50
    LBM-Broth Low Salt
    For propagation of of lambda phages in Escherichia coli.In LBM media Mg2 is added to enhance adsorbtion of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption.
  • LBM-Agar Lennox

    £10.58£194.22
    SKU Pack Size Price
    LXM0201250g £10.58
    LXM02021000g £35.95
    LXM02036 x 1kg £194.22
    LBM-Agar Low Salt
    For propagation of of lambda phages in Escherichia coli.In LBM media Mg2 is added to enhance adsorbtion of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption.
  • LB Broth Miller

    £2.56£185.88
    SKU Pack Size Price
    LMM1L1 Litre pack - 25g £2.56
    LMM2L2 Litre pack - 50g £3.57
    LMM5L5 Litre pack - 125 gram £7.02
    LMM10L10 Litre pack - 250g £11.91
    LMM20L20 Litre pack - 500g £22.26
    LMM0101250g £11.91
    LMM0104500g £22.26
    LMM01021000g £36.96
    LMM01051500g £52.65
    LMM01036 x 1kg £185.88
    Suspend 25 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • LB-Agar Miller

    £3.23£265.57
    SKU Pack Size Price
    LMM02-1L1 Litre pack £3.23
    LMM02-2L2 Litre pack £4.90
    LMM02-5L5 Litre pack £11.47
    LMM02-10L10L pack £21.04
    LMM02-20L20 Litre pack £39.74
    LMM0201250g £15.48
    LMM0204500g £28.28
    LMM02021000g £49.09
    LMM02036 x 1kg £265.57
    LB-Agar High Salt, Miller's LB Broth, Luria Agar
  • LB-Top Agar Miller

    £16.14£51.54
    SKU Pack Size Price
    LMM0301250g £16.14
    LMM03021000g £51.54
  • LBM-Broth Miller

    £11.91£200.01
    SKU Pack Size Price
    LMG0101250g £11.91
    LMG01021000g £37.07
    LMG01036 x 1kg £200.01
    LBM-Broth High Salt
    In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption.
  • LBM-Agar Miller

    £16.03£278.81
    SKU Pack Size Price
    LMG0201250g £16.03
    LMG02021000g £51.54
    LMG02036 x 1kg £278.81
    LBM-Agar Low Salt
    In LBM media Mg2 is added to enhance adsorbtion of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption.
  • 2X LB-Broth

    £8.80£182.54
    SKU Pack Size Price
    LBD0101250g £8.80
    LBD01021000g £33.95
    LBD01036 x 1kg £182.54
    For maintenance and propagation of Escherichia coli.
  • 2X LB-Agar

    £10.58£220.94
    SKU Pack Size Price
    LBD0201250g £10.58
    LBD02021000g £40.30
    LBD02036 x 1kg £220.94
    For maintenance and propagation of Escherichia coli
  • Nutrient Broth

    £29.28£290.28
    SKU Pack Size Price
    NBO01500g £29.28
    NBO021000g £53.76
    NBO036 x 1kg £290.28
  • Nutrient Agar

    £31.61£321.88
    SKU Pack Size Price
    NAO01500g £31.61
    NAO021000g £59.66
    NAO036 x 1kg £321.88
  • Tryptone Broth

    £12.03£237.07
    SKU Pack Size Price
    TPB0101250g £12.03
    TPB01021000g £43.97
    TPB01036 x 1kg £237.07
    General purpose medium for maintenance and propagation of Escherichia coli.
  • Tryptone Agar

    £15.70£311.09
    SKU Pack Size Price
    TPA0101250g £15.70
    TPA01021000g £57.77
    TPA01036 x 1kg £311.09
  • Tryptone Top Agar

    £15.55£57.77
    SKU Pack Size Price
    TPT0101250g £15.55
    TPT01021000g £57.77
  • Terrific Broth

    £12.03£237.63
    SKU Pack Size Price
    TRB0101250g £12.03
    TRB0104500g £21.71
    TRB01021000g £43.97
    TRB01036 x 1kg £237.63
    Terrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E.coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium.Terrific medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid.Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen, vitamins and cofactors for a luxurious growth.Potassium phosphate is present in Terrific broth buffered to balance the pH and subsequently prevent cell death due to a drop in pH.Tartoff, K. D., and C. A. Hobbs, Improved media for growing plasmids and cosmid clones, Bethesda Research Laboratories, 9, 12, (1987). Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
  • Terrific Agar

    £15.44£282.69
    SKU Pack Size Price
    TRA0101250g £15.44
    TRA01021000g £52.48
    TRA01036 x 1kg £282.69
  • SKU Pack Size Price
    TBP0101250g £12.03
    TBP01021kg £43.97
    TBP01036 x 1kg £237.63
    Phosphate Buffered
  • SKU Pack Size Price
    TAP0101250g £14.47
    TAP01021000g £52.87
    TAP01036 x 1kg £285.38
    Phosphate Buffered
  • Super Broth

    £14.47£285.82
    SKU Pack Size Price
    SPB0101250g £14.47
    SPB01021000g £52.98
    SPB01036 x 1kg £285.82
    Super broth is an extremely rich medium with highly elevated levels of Tryptone and Yeast extract. Due to the high levels of peptides, amino acids, vitamins and cofactors high yields of E. coli cells and lambda bacteriophages are obtained.
  • Super Broth Agar

    £16.59£328.01
    SKU Pack Size Price
    SPA0101250g £16.59
    SPA01021000g £60.89
    SPA01036 x 1kg £328.01
  • Super Broth Top Agar

    £16.59£60.77
    SKU Pack Size Price
    SPT0101250g £16.59
    SPT01021000g £60.77
  • M9 Minimal Salts Base, 5x

    £21.39£369.34
    SKU Pack Size Price
    MMS0101250g £21.39
    MMS01021000g £68.91
    MMS01036 x 1kg £369.34
    M9 Minimal Salts Base, 5x is used in preparing M9 Minimal medium used for the cultivation and maintenance of E. coli in molecular biology.M9 is a chemical defined minimal medium supporting the growth of  �wild-type� strains of E.coli. The medium can be supplemented with specific amino acids or other required nutrients allowing for selection of specific auxotrophs.Glucose may be added as a source of carbohydrate. Nitrogen is provided by ammonium chloride final osmolarity of the medium is maintained by NaCl. Addition of magnesium and calcium increases the growth of the recombinant cells.M9 Minimal Salts Base, 5x is a 5x concentrate and has to be diluted to a 1x concentration and subsequently supplemented with an appropriate carbon source, such as glucose. Addition of required nutritionally elements may be added.Difco Manual, 11th edition, Sparks, MD, 272, 1998. Davis, L.G., M.D. Dibner and J.F. Battey, Basic methods in molecular biology, Elsevier, new York, (1986). Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
  • M17 Broth

    £92.38£504.86
    SKU Pack Size Price
    M1701101kg £92.38
    M1701606 x 1kg £504.86
    M17 Medium is a well buffered enriched medium for bacterial growth. The medium is optimized for isolating lactic Streptococci. Casein hydrolysate, Beef extract and Soya peptone supply carbon sources, nitrogen, vitamins and minerals essential for vigorous growth. Yeast extract supplies B-complex vitamins which stimulate bacteria growth. The medium is buffered by Disodium-β-glycerophosphate which prevents a drop in pH by the production of organic acids, such as lactic acid, during fermentation. Ascorbic acid and Magnesium sulphate are added to stimulate growth.
  • Blood Agar Base

    £39.74£387.11
    SKU Pack Size Price
    BAB0105500g £39.74
    BAB01101000g £70.12
    BAB01606 x 1kg £387.11
  • Malt Extract Agar

    £22.26£390.67
    SKU Pack Size Price
    MEA0102250g £22.26
    MEA01101000g £72.35
    MEA01606 x 1kg £390.67
  • MacConkey Agar Base

    £116.87£631.08
    SKU Pack Size Price
    MAB101000g £116.87
    MAB606 x 1kg £631.08
  • Mueller Hinton Broth

    £31.17£480.82
    SKU Pack Size Price
    MHB0100250g £31.17
    MHB01011000g £89.04
    MHB01026 x 1kg £480.82