M13 Phage and ssDNA Bacteriophages Medium Formulations

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M13 Phage and ssDNA Bacteriophages Medium Formulations

YT and 2X YT are designed for cultivation of recombinant strains of Escherichia Coli . These media are also used for maintenance and propagation of M13 bacteriophage and several types of ssDNA bacteriophage.

YT is a moderately rich medium, where 2x YT is a nutritional rich medium providing nitrogen and growth factors allowing bacteriophage to reproduce in large quantities without exhausting the host cells.  The presence of amino acids, nucleotides, vitamins and other nutritional elements, that the cell would otherwise have to synthesize, stimulates a luxurious growth and high yields of the host cells.  Sodium Chloride is present in the medium to provide a suitable osmolarity.

Difco manual 11th ed., Sparks, MD (1998), 22-23.
Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).
Davis, L.G., M.D. Dibner and J.F. Battey, Basic methods in molecular biology, Elsevier, new York, (1986).

  • YT Broth

    £11.69£223.83
    SKU Pack Size Price
    YTB0101250g £11.69
    YTB01021000g £41.52
    YTB01036 x 1kg £223.83
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    Suspend 18 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • YT Agar

    £15.59£298.18
    SKU Pack Size Price
    YTA0201250g £15.59
    YTA02021000g £55.21
    YTA02036 x 1kg £298.18
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    Formula
    g/l
    Tryptone8
    Yeast extract5
    NaCl5
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 33 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • YT Top Agar

    £14.14£44.64
    SKU Pack Size Price
    YTT0301250g £14.14
    YTT03021000g £44.64
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    Formula
    g/l
    Tryptone8
    Yeast extract5
    NaCl5
    Agar7
    Final pH7.0 ± 0.2 at 25°C
    Suspend 25 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • 2X YT Broth

    £3.23£244.86
    SKU Pack Size Price
    YDB1L1 Litre pack - 31g £3.23
    YDB2L2 Litre pack £4.90
    YDB5L5 Litre pack £8.24
    YDB10L10 Litre pack - 310g £15.81
    YDB0101250g £12.92
    YDB20L20 Litre pack £30.39
    YDB01021000g £44.75
    YDB01036 x 1kg £244.86
    Select options
    Formula
    g/l
    Tryptone16
    Yeast extract10
    NaCl5
    Final pH7.0 ± 0.2 at 25°C
    Suspend 31 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • 2X YT Agar

    £4.23£306.41
    SKU Pack Size Price
    YDA1L1 Litre pack £4.23
    YDA2L2 Litre pack £6.35
    YDA5L5 Litre pack £14.70
    YDA10L10L pack £28.05
    YDA20L20 Litre pack £53.76
    YDA0201250g £15.92
    YDA02021000g £56.66
    YDA02036 x 1kg £306.41
    Select options
    Formula
    g/l
    Tryptone16
    Yeast extract10
    NaCl5
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 46 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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