Bacteriophage Lambda Medium Formulations

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Bacteriophage Lambda Medium Formulations

Formedium™ manufactures a large range of media for maintenance and propagation of bacteriophage lambda in Escherichia Coli cells as described by Blattner, F. et al., Science, 196, 161, 1977.

NZCYM medium, as originally developed by Blattner, is available in several modification providing the researcher to select the optimum medium formulation for a specific strain.

Tryptone or Casein, Yeast Extract and Casamino acids are sources of nitrogen within this group of media.  Peptides and amino acids are abundantly present in Tryptone. Yeast Extract is a abundantly source of amino acids, vitamins, nucleotides and carbohydrates.  Due to its high degree of digestion Casamino acids is a rich source of free amino acids.

Sodium ions for transport and a suitable osmotic balance are provided by Sodium Chloride.

Magnesium Sulfate provides magnesium ions required in a variety of enzymatic reactions, including DNA replication.

To optimise binding of phage lambda to Escherichia Coli cells add 0.2% Maltose to the medium for inducing lambda receptors (LampB) on host cells.

Blattner, F.R., et al., Charon phages: Safer derivatives of bateriophage for DNA cloning, Science, 196, 161, (1977).
Difco manual 11th ed., Sparks, MD (1998), 22-23.
Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).

  • NZ Broth

    £15.03£38.96
    SKU Pack Size Price
    NZB0101250g £15.03
    NZB01021000g £38.96
    NZM Broth
    Select options
    Formula
    g/l
    Tryptone10
    NaCl5
    MgSO4 anhydrous0.98
    Final pH7.0 ± 0.2 at 25°C
    Suspend 16 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • NZ Agar

    £16.70£51.20
    SKU Pack Size Price
    NZA0101250g £16.70
    NZA01021000g £51.20
    NZM Agar
    Select options
    Formula
    g/l
    Tryptone10
    NaCl5
    MgSO4 anhydrous0.98
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 31 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • NZ Top Agar

    £16.70£51.20
    SKU Pack Size Price
    NZT0301250g £16.70
    NZT03021000g £51.20
    NZM Top Agar
    Select options
    Formula
    g/l
    Tryptone10
    NaCl5
    MgSO4 anhydrous0.98
    Agar7
    Final pH7.0 ± 0.2 at 25°C
    Suspend 23 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • NZCYM Broth

    £13.03£215.70
    SKU Pack Size Price
    NZC0101250g £13.03
    NZC01021000g £38.96
    NZC01036 x 1kg £215.70
    Select options
    Formula
    g/l
    Casamino acids1
    Tryptone10
    Yeast extract5
    NaCl5
    MgSO4 anhydrous0.98
    Final pH7.0 ± 0.2 at 25°C
    Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • NZCYM Agar

    £18.48£305.41
    SKU Pack Size Price
    NZC0201250g £18.48
    NZC02021000g £55.32
    NZC02036 x 1kg £305.41
    Select options
    Formula
    g/l
    Casamino acids1
    Tryptone10
    Yeast extract5
    NaCl5
    MgSO4 anhydrous0.98
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 37 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • NZCYM Top Agar

    £17.15£51.43
    SKU Pack Size Price
    NZC0301250g £17.15
    NZC03021000g £51.43
    Select options
    Formula
    g/l
    Casamino acids1
    Tryptone10
    Yeast extract5
    NaCl5
    MgSO4 anhydrous0.98
    Agar7
    Final pH7.0 ± 0.2 at 25°C
    Suspend 29 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZY Broth

    £13.03£44.86
    SKU Pack Size Price
    NZY0101250g £13.03
    NZY01021000g £44.86
    Harvard Broth
    Select options
    Formula
    g/l
    Tryptone10
    Yeast extract5
    NaCl8
    Final pH7.0 ± 0.2 at 25°C
    Suspend 23 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • NZY Agar

    £17.26£58.99
    SKU Pack Size Price
    NZY0201250g £17.26
    NZY02021000g £58.99
    Harvard Agar
    Select options
    Formula
    g/l
    Tryptone10
    Yeast extract5
    NaCl8
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 38 gram powdered medium in 1 litre distilled water Store dry at room temperature.
    View product
  • SKU Pack Size Price
    NZD0101500g £47.31
    Select options
    Formula
    g/l
    Tryptone10
    Yeast extract5
    NaCl5
    MgSO4 anhydrous0.49
    Thymidine0.04
    Final pH7.0 ± 0.2 at 25°C
     Suspend 20.5 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • SKU Pack Size Price
    NZD0201500g £60.66
    Select options
    Formula
    g/l
    Tryptone10
    Yeast extract5
    NaCl5
    MgSO4 anhydrous0.49
    Thymidine0.04
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • NZYM Broth

    £13.69£240.75
    SKU Pack Size Price
    NZM0101250g £13.69
    NZM01021000g £43.63
    NZM01036 x 1kg £240.75
    NZY Broth
    Select options
    Formula
    g/l
    Tryptone10
    Yeast extract5
    NaCl5
    MgSO4 anhydrous0.98
    Final pH7.0 ± 0.2 at 25°C
    Suspend 21 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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  • NZYM Agar

    £17.70£310.75
    SKU Pack Size Price
    NZM0201250g £17.70
    NZM02021000g £56.21
    NZM02036 x 1kg £310.75
    NZY Agar
    Select options
    Formula
    g/l
    Tryptone10
    Yeast extract5
    NaCl5
    MgSO4 anhydrous0.98
    Agar15
    Final pH7.0 ± 0.2 at 25°C
    Suspend 36 gram powdered medium in 1 litre distilled water Store dry at room temperature.
    View product
  • NZYM Top Agar

    £15.92£51.31
    SKU Pack Size Price
    NZM0301250g £15.92
    NZM03021000g £51.31
    NZY Top Agar
    Select options
    Formula
    g/l
    Tryptone10
    Yeast extract5
    NaCl5
    MgSO4 anhydrous0.98
    Agar7
    Final pH7.0 ± 0.2 at 25°C
    Suspend 28 gram powdered medium in 1 litre distilled water Store dry at room temperature.
    View product