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Bacteriophage Lambda Medium Formulations

Formedium™ manufactures a large range of media for maintenance and propagation of bacteriophage lambda in Escherichia Coli cells as described by Blattner, F. et al., Science, 196, 161, 1977.

NZCYM medium, as originally developed by Blattner, is available in several modification providing the researcher to select the optimum medium formulation for a specific strain.

Tryptone or Casein, Yeast Extract and Casamino acids are sources of nitrogen within this group of media.  Peptides and amino acids are abundantly present in Tryptone. Yeast Extract is a abundantly source of amino acids, vitamins, nucleotides and carbohydrates.  Due to its high degree of digestion Casamino acids is a rich source of free amino acids.

Sodium ions for transport and a suitable osmotic balance are provided by Sodium Chloride.

Magnesium Sulfate provides magnesium ions required in a variety of enzymatic reactions, including DNA replication.

To optimise binding of phage lambda to Escherichia Coli cells add 0.2% Maltose to the medium for inducing lambda receptors (LampB) on host cells.

Blattner, F.R., et al., Charon phages: Safer derivatives of bateriophage for DNA cloning, Science, 196, 161, (1977).
Difco manual 11th ed., Sparks, MD (1998), 22-23.
Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).

  • NZ Broth

    $23.39$60.64
    SKU Pack Size Price
    NZB0101 250g $23.39
    NZB0102 1000g $60.64
    NZM Broth
    Select options
    Formula
    g/l
    Tryptone 10
    NaCl 5
    MgSO4 anhydrous 0.98
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 16 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZ Agar

    $25.99$79.70
    SKU Pack Size Price
    NZA0101 250g $25.99
    NZA0102 1000g $79.70
    NZM Agar
    Select options
    Formula
    g/l
    Tryptone 10
    NaCl 5
    MgSO4 anhydrous 0.98
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 31 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZ Top Agar

    $25.99$79.70
    SKU Pack Size Price
    NZT0301 250g $25.99
    NZT0302 1000g $79.70
    NZM Top Agar
    Select options
    Formula
    g/l
    Tryptone 10
    NaCl 5
    MgSO4 anhydrous 0.98
    Agar 7
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 23 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZCYM Broth

    $20.27$335.76
    SKU Pack Size Price
    NZC0101 250g $20.27
    NZC0102 1000g $60.64
    NZC0103 6 x 1kg $335.76
    Select options
    Formula
    g/l
    Casamino acids 1
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.98
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZCYM Agar

    $28.76$475.40
    SKU Pack Size Price
    NZC0201 250g $28.76
    NZC0202 1000g $86.11
    NZC0203 6 x 1kg $475.40
    Select options
    Formula
    g/l
    Casamino acids 1
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.98
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 37 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZCYM Top Agar

    $26.68$80.04
    SKU Pack Size Price
    NZC0301 250g $26.68
    NZC0302 1000g $80.04
    Select options
    Formula
    g/l
    Casamino acids 1
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.98
    Agar 7
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 29 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZY Broth

    $20.27$69.82
    SKU Pack Size Price
    NZY0101 250g $20.27
    NZY0102 1000g $69.82
    Harvard Broth
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 8
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 23 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZY Agar

    $26.85$91.82
    SKU Pack Size Price
    NZY0201 250g $26.85
    NZY0202 1000g $91.82
    Harvard Agar
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 8
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 38 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    NZD0101 500g $73.63
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.49
    Thymidine 0.04
    Final pH 7.0 ± 0.2 at 25°C
      Suspend 20.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    NZD0201 500g $94.42
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.49
    Thymidine 0.04
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • NZYM Broth

    $21.31$374.74
    SKU Pack Size Price
    NZM0101 250g $21.31
    NZM0102 1000g $67.91
    NZM0103 6 x 1kg $374.74
    NZY Broth
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.98
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 21 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • NZYM Agar

    $27.55$483.71
    SKU Pack Size Price
    NZM0201 250g $27.55
    NZM0202 1000g $87.49
    NZM0203 6 x 1kg $483.71
    NZY Agar
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.98
    Agar 15
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 36 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • NZYM Top Agar

    $24.77$79.87
    SKU Pack Size Price
    NZM0301 250g $24.77
    NZM0302 1000g $79.87
    NZY Top Agar
    Select options
    Formula
    g/l
    Tryptone 10
    Yeast extract 5
    NaCl 5
    MgSO4 anhydrous 0.98
    Agar 7
    Final pH 7.0 ± 0.2 at 25°C
    Suspend 28 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product