SIH Defined Minimal Media
Dictyostelium Discoideum
SIH Defined Minimal Media
Synthetic Defined Minimal Media
This media is based on well defined formulations of mineral salts, vitamins and amino acids.
Two commonly used synthetic defined minimal Dictyostelium Discoideum media are FM Media and SIH Media.
FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.
SIH medium is a newly developed modification of FM with regards to its amino acid compostion. Aspartic acid is added. Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.
Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins. Besides these components, there is a large group of amino acids present. To facilitate genetic studies ForMedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.
New to our range of synthetic media are FM and SIH without all amino acids. These two newly developed media, combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.
Improvement of a synthetic medium for Dictyostelium discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004
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SKU Size Price SIH0101 250g $67.63 SIH0102 1kg $225.29 SIH0103 6 x 1kg $1,216.46 SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1.1 mM) and increased levels of Lysine (8.5 mM) and Tryptophane (1.7 mM). Concentrations of Cysteine, Glutamic acid, Methionine, Phenylalanine, Threonine are slightly increased as well in SIH. The alterations in amino acid formulation of SIH medium resulted in a more even and better amino acid utilisation. Cell density of D. discoideum rose up to levels in excess of 5 x 107 cells compared to FM medium with cell density levels of 3 x 107. Improvement of a synthetic medium for Dictyostelium discoideum, Sang-In Han, Karl Friehs and Erwin Flaschel, Process Biochemistry, 39 (8), 925 - 930, 2004. Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor. Sang-In Han, Karl Friehs and Erwin Flaschel, Process Biochemistry, 39, 585 - 589, 2004. -
SKU Size Price SHA02 100g $67.63 SHA03 250g $135.17 SIH Amino acid drop-out mixture is based on the Amino acids present in SIH Minimal medium. These Amino acids form a basis for many "Drop-Out" mixtures to select for auxotrophic requirements and transformants. Each SIH Drop-Out mixture contain all components except for one or two essential Amino acids, i.e. the "dropped out" supplements. SIH Amino acids drop-out mixtures are used in combination with SIH Minimal medium w/o Amino acids or SIH Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium. Complete Supplement Mixture formulations are available in 25 gram and 250 gram pack sizes. Please enquire about custom made formulations.