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Non Defined Complex Dictyostelium Discoideum

Some specific strains of Dictyostelium Discoideum are capable to grow axenically in liquid media without bacteria as food. Peptone and Yeast Extract are the main components of these media within this group. Both products are present in various ratio’s in different  Dicty media.  Peptone provides high molecular weight peptides and proteins as a nitrogen source.  Yeast Extract is a source of vitamins, co-factors and carbohydrates. Often there is an additional Phosphate buffer present to inhibit acidification of the medium during cell growth.

Commonly used HL5 and HL5-C media are also available supplemented with extra vitamins and micro-elements. This supplement  gives good results for cultures of Dicty cellines who require additional nutritional elements for starting up vigorous growth or protein synthesis.

  • A Medium

    £17.59£284.04
    SKU Pack Size Price
    AMD0101250g £17.59
    AMD01036 x 1kg £284.04
    AMD01021kg £52.65
    Select options
    Formula
    g/l
    Peptone5
    Yeast Extract0.5
    Glucose5
    KH2PO42.25
    K2HPO40.7
    MgSO4.anhydrous0.25
     Suspend 13.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • AX Medium

    £17.59£284.04
    SKU Pack Size Price
    AXM0101250g £17.59
    AXM01036 x 1kg £284.04
    AXM01021000g £52.65
    Select options
    Formula
    g/l
    Peptone14.3
    Yeast Extract7.15
    Glucose18
    KH2PO40.49
    Na2HPO4.anhydrous0.49
     Suspend 40.4 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Broth

    £18.70£302.96
    SKU Pack Size Price
    SMB01021000g £56.10
    SMB01036 x 1kg £302.96
    SMB0101250g £18.70
    Select options
    Formula
    g/l
    Peptone10
    Yeast Extract1
    Glucose10
    KH2PO41.9
    K2HPO4.3H2O1.3
    MgO4.anhydrous0.49
     Suspend 24.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Agar

    £19.93£359.73
    SKU Pack Size Price
    SMA0101250g £19.93
    SMA01036 x 1kg £359.73
    SMA01021000g £66.67
    Select options
    Formula
    g/l
    Peptone10
    Yeast Extract1
    Glucose10
    KH2PO41.9
    K2HPO4.3H2O1.3
    MgSO4.anhydrous0.49
    Agar17
     Suspend 41.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Broth/5

    £18.70£302.96
    SKU Pack Size Price
    SMB501036 x 1kg £302.96
    SMB501021000g £56.10
    SMB50101250g £18.70
    Select options
    Formula
    g/l
    Peptone2
    Yeast Extract0.2
    Glucose0.2
    KH2PO41.9
    K2HPO4.3H2O1.3
    MgO4.anhydrous0.49
     Suspend 7.89 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Agar/5

    £19.93£359.73
    SKU Pack Size Price
    SMA501036 x 1kg £359.73
    SMA501021000g £66.67
    SMA50101250g £19.93
    Select options
    Formula
    g/l
    Peptone2
    Yeast Extract0.2
    Glucose2
    KH2PO41.9
    K2HPO4.3H2O1.3
    MgO4.anhydrous0.49
    Agar17
     Suspend 24.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    VL60101250g £16.54
    VL601021kg £55.13
    VL601036 x 1kg £248.07
    VL6 is a complex medium for cultivation of Discoideum dictyostelium based on Vegetable Peptone.Formedium Ltd have recognised the need for a range of meat-free products. This has led to the development of VL6, an animal component free alternative medium compared to traditional Dicty media such as HL5 and HL5C.VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source.  Yeast Extract is a source of vitamins, co-factors and carbohydrates. A Phosphate buffer is present to inhibit acidification of the medium during cell growth.As most vegetable peptones contain almost no Tryptophane VL6 is supplemented with an extra quantity of this amino acid and some other amino acids. Essential trace elements and vitamins as present in FM and SIH media are added for those Dicty cultures needing additional nutritionals for starting up vigorous growth or protein synthesis.
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    Suspend 35.95g in 1L of Distilled or De-ionised Water
    Components
     
    Vegetable Peptone10g/L
    Yeast extract7g/L
    Potassium Dihydrogen Phosphate1.2g/L
    Disodium Hydrogen Phosphate0.35g/L
    Glucose12g/L
    Trace elements0.1g/L
    VL6 Amino acid supplement5.3g/L
    35.95
     The materials used in the production are in compliance with the European Directive 75/318/EEC as amended by Directive 1999/82/EC. Therefore Formedium takes the position that this product is free of any risk in terms of Bovine Spongiform Encephalothy (BSE) or Transmissibe Spongiform Encephalopathy (TSE).HYGROSCOPIC Store at Room Temperature DO NOT BREATHE DUST Keep Container Tightly Closed 
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  • SKU Pack Size Price
    VL60201250g £19.85
    VL602021kg £66.15
    VL602036 x 1kg £357.21
    VL6 is a complex medium for cultivation of Discoideum dictyostelium based on Vegetable Peptone.Formedium Ltd have recognised the need for a range of meat-free products. This has led to the development of VL6, an animal component free alternative medium compared to traditional Dicty media such as HL5 and HL5C.VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source. Yeast Extract is a source of vitamins, co-factors and carbohydrates. A Phosphate buffer is present to inhibit acidification of the medium during cell growth.As most vegetable peptones contain almost no Tryptophane VL6 is supplemented with an extra quantity of this amino acid and some other amino acids. Essential trace elements and vitamins as present in FM and SIH media are added for those Dicty cultures needing additional nutritionals for starting up vigorous growth or protein synthesis.
    Select options
    Suspend 23.95g in 1L of Distilled or De-ionised Water
    Components
     
    Vegetable Peptone10g/L
    Yeast extract7g/L
    Potassium Dihydrogen Phosphate1.2g/L
    Disodium Hydrogen Phosphate0.35g/L
    Trace elements0.1g/L
    VL6 Amino acid supplement5.3g/L
    23.95
     The materials used in the production are in compliance with the European Directive 75/318/EEC as amended by Directive 1999/82/EC. Therefore Formedium takes the position that this product is free of any risk in terms of Bovine Spongiform Encephalothy (BSE) or Transmissibe Spongiform Encephalopathy (TSE).HYGROSCOPIC Store at Room Temperature DO NOT BREATHE DUST Keep Container Tightly Closed 
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  • HL5 Medium including Glucose

    £16.37£296.62
    SKU Pack Size Price
    HLG0101250g £16.37
    HLG01021000g £53.76
    HLG01036 x 1kg £296.62
    Select options
    Formula
    g/l
    Peptone14
    Yeast Extract7
    Glucose13.5
    KH2PO40.5
    Na2HPO40.5
     Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • HL5 Medium without Glucose

    £17.59£375.75
    SKU Pack Size Price
    HLB0101250g £17.59
    HLB01021000g £66.67
    HLB01036 x 1kg £375.75
    Select options
    Formula
    g/l
    Peptone14
    Yeast Extract7
    KH2PO40.5
    Na2HPO40.5
     Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLC0101250g £16.37
    HLC01036 x 1kg £290.28
    HLC01021000g £53.76
    Select options
    Formula
    g/l
    Peptone5
    Yeast Extract5
    Tryptone5
    KH2PO41.2
    Na2HPO40.35
    Glucose10
     Suspend 26.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • HL5-C Medium without Glucose

    £17.59£354.83
    SKU Pack Size Price
    HLD0101250g £17.59
    HLD01021000g £64.34
    HLD01036 x 1kg £354.83
    Select options
    Formula
    g/l
    Peptone5
    Yeast Extract5
    Tryptone5
    KH2PO41.2
    Na2HPO40.35
     Suspend 16.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLE1250g £16.92
    HLE21000g £56.10
    HLE36 x 1kg £302.96
    HL5 supplemented with vitamins and micro-elements as present in FM and SIH media.The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis
    Select options
    Formula
    g/l
    Peptone14
    Yeast Extract7
    Glucose13.5
    KH2PO40.5
    Na2HPO40.5
    FM Vitamins and Micro-elements0.01
     Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLF1250g £18.15
    HLF21000g £67.79
    HLF36 x 1kg £366.07
    HL5 Medium supplemented with vitamins and micro-elements as present in FM and SIH media.The addition of extra vitamins and micro-elements gives good results for cultures of Dictyostelium Discoideum who require additional nutritional elements for starting up vigorous growth or protein synthesis.
    Select options
    Formula
    g/l
    Peptone14
    Yeast Extract7
    KH2PO40.5
    Na2HPO40.5
    FM Vitamins and Micro-elements0.01
     Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • SKU Pack Size Price
    HLH1250g £16.92
    HLH21kg £54.99
    HLH36 x 1kg £296.62
    HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media.The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.
    Select options
    Formula
    g/l
    Peptone5
    Yeast Extract5
    Tryptone5
    KH2PO41.2
    Na2HPO40.35
    Glucose10
    FM Vitamins and Micro-elements0.01
     Suspend 26.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
    View product
  • SKU Pack Size Price
    HLI1250g £18.15
    HLI21kg £65.45
    HLI36 x 1kg £361.28
    HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media.The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.
    Select options
    Formula
    g/l
    Peptone5
    Yeast Extract5
    Tryptone5
    KH2PO41.2
    Na2HPO40.35
    FM Vitamins and Micro-elements0.01
     Suspend 16.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LoFlo Medium

    £37.40£353.38
    SKU Pack Size Price
    LF0501500g £37.40
    LF60016 x 1kg £353.38
    LF10011kg £65.45
    Select options
    Formula
    mg/l
    Glucose11000 g/l
    KH2PO4680.45 g/l
    Casein Peptone5000 g/l
    NH4Cl26.8
    MgCl237.1
    CaCl21.1
    FeCl38.11
    Na2-EDTA4.84
    ZnSO42.30
    H3BO31.11
    MnCl2.4H2O0.51
    CoCl20.17
    CuSO5.5H2O0.15
    (NH4)6M07O24.4H2O0.1
     Suspend 16.8 gram powdered medium in 1 litre distilled water Store dry at room temperature Adjust to pH 6.5
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  • SKU Pack Size Price
    LFG0501500g £37.40
    LFG60016 x 1kg £353.38
    LFG10011kg £65.45
    Select options
    Formula
    g/l
    Glucose11
    KH2PO40.68
    Casein Peptone5
    Yeast extract0.7
    NH4Cl26.8
    MgCl237.1
    CaCl21.1
    FeCl38.11
    Na2-EDTA4.84
    ZnSO42.30
    H3BO31.11
    MnCl2.4H2O0.51
    CoCl20.17
    CuSO5.5H2O0.15
    (NH4)6M07O24.4H2O0.1
     Suspend 17.5 gram powdered medium in 1 litre distilled water Store dry at room temperature Adjust to pH 6.5
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