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Non Defined Complex Dictyostelium Discoideum

Some specific strains of Dictyostelium Discoideum are capable to grow axenically in liquid media without bacteria as food. Peptone and Yeast Extract are the main components of these media within this group. Both products are present in various ratio’s in different  Dicty media.  Peptone provides high molecular weight peptides and proteins as a nitrogen source.  Yeast Extract is a source of vitamins, co-factors and carbohydrates. Often there is an additional Phosphate buffer present to inhibit acidification of the medium during cell growth.

Commonly used HL5 and HL5-C media are also available supplemented with extra vitamins and micro-elements. This supplement  gives good results for cultures of Dicty cellines who require additional nutritional elements for starting up vigorous growth or protein synthesis.

  • A Medium

    $27.37$442.13
    SKU Pack Size Price
    AMD0101 250g $27.37
    AMD0103 6 x 1kg $442.13
    AMD0102 1kg $81.95
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    Formula
    g/l
    Peptone 5
    Yeast Extract 0.5
    Glucose 5
    KH2PO4 2.25
    K2HPO4 0.7
    MgSO4.anhydrous 0.25
      Suspend 13.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • AX Medium

    $27.37$442.13
    SKU Pack Size Price
    AXM0101 250g $27.37
    AXM0103 6 x 1kg $442.13
    AXM0102 1000g $81.95
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    Formula
    g/l
    Peptone 14.3
    Yeast Extract 7.15
    Glucose 18
    KH2PO4 0.49
    Na2HPO4.anhydrous 0.49
      Suspend 40.4 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Broth

    $29.11$471.59
    SKU Pack Size Price
    SMB0102 1000g $87.32
    SMB0103 6 x 1kg $471.59
    SMB0101 250g $29.11
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    Formula
    g/l
    Peptone 10
    Yeast Extract 1
    Glucose 10
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
      Suspend 24.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Agar

    $31.01$559.94
    SKU Pack Size Price
    SMA0101 250g $31.01
    SMA0103 6 x 1kg $559.94
    SMA0102 1000g $103.78
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    Formula
    g/l
    Peptone 10
    Yeast Extract 1
    Glucose 10
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgSO4.anhydrous 0.49
    Agar 17
      Suspend 41.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Broth/5

    $29.11$471.59
    SKU Pack Size Price
    SMB50103 6 x 1kg $471.59
    SMB50102 1000g $87.32
    SMB50101 250g $29.11
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    Formula
    g/l
    Peptone 2
    Yeast Extract 0.2
    Glucose 0.2
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
      Suspend 7.89 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SM Agar/5

    $31.01$559.94
    SKU Pack Size Price
    SMA50103 6 x 1kg $559.94
    SMA50102 1000g $103.78
    SMA50101 250g $31.01
    Select options
    Formula
    g/l
    Peptone 2
    Yeast Extract 0.2
    Glucose 2
    KH2PO4 1.9
    K2HPO4.3H2O 1.3
    MgO4.anhydrous 0.49
    Agar 17
      Suspend 24.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • HL5 Medium without Glucose

    $27.37$584.89
    SKU Pack Size Price
    HLB0101 250g $27.37
    HLB0103 6 x 1kg $584.89
    HLB0102 1000g $103.78
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    KH2PO4 0.5
    Na2HPO4 0.5
      Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLG0101 250g $25.47
    HLG0103 6 x 1kg $461.71
    HLG0102 1000g $83.68
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    Glucose 13.5
    KH2PO4 0.5
    Na2HPO4 0.5
      Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLC0101 250g $25.47
    HLC0103 6 x 1kg $451.84
    HLC0102 1000g $83.68
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    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    Glucose 10
      Suspend 26.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLD0102 1000g $100.14
    HLD0103 6 x 1kg $552.32
    HLD0101 250g $27.37
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    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
      Suspend 16.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLE2 1000g $87.32
    HLE3 6 x 1kg $471.59
    HLE1 250g $26.33
    HL5 supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis
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    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    Glucose 13.5
    KH2PO4 0.5
    Na2HPO4 0.5
    FM Vitamins and Micro-elements 0.01
      Suspend 35.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLF1 250g $28.24
    HLF2 1000g $105.51
    HLF3 6 x 1kg $569.82
    HL5 Medium supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dictyostelium Discoideum who require additional nutritional elements for starting up vigorous growth or protein synthesis.
    Select options
    Formula
    g/l
    Peptone 14
    Yeast Extract 7
    KH2PO4 0.5
    Na2HPO4 0.5
    FM Vitamins and Micro-elements 0.01
      Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLH1 250g $26.33
    HLH3 6 x 1kg $461.71
    HLH2 1kg $85.59
    HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.
    Select options
    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    Glucose 10
    FM Vitamins and Micro-elements 0.01
      Suspend 26.5 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • SKU Pack Size Price
    HLI2 1kg $101.87
    HLI1 250g $28.24
    HLI3 6 x 1kg $562.37
    HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media. The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.
    Select options
    Formula
    g/l
    Peptone 5
    Yeast Extract 5
    Tryptone 5
    KH2PO4 1.2
    Na2HPO4 0.35
    FM Vitamins and Micro-elements 0.01
      Suspend 16.55 gram powdered medium in 1 litre distilled water Store dry at room temperature
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  • LoFlo Medium

    $58.21$550.07
    SKU Pack Size Price
    LF0501 500g $58.21
    LF6001 6 x 1kg $550.07
    LF1001 1kg $101.87
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    Formula
    mg/l
    Glucose 11000 g/l
    KH2PO4 680.45 g/l
    Casein Peptone 5000 g/l
    NH4Cl 26.8
    MgCl2 37.1
    CaCl2 1.1
    FeCl3 8.11
    Na2-EDTA 4.84
    ZnSO4 2.30
    H3BO3 1.11
    MnCl2.4H2O 0.51
    CoCl2 0.17
    CuSO5.5H2O 0.15
    (NH4)6M07O24.4H2O 0.1
      Suspend 16.8 gram powdered medium in 1 litre distilled water Store dry at room temperature Adjust to pH 6.5
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  • SKU Pack Size Price
    LFG0501 500g $58.21
    LFG6001 6 x 1kg $550.07
    LFG1001 1kg $101.87
    Select options
    Formula
    g/l
    Glucose 11
    KH2PO4 0.68
    Casein Peptone 5
    Yeast extract 0.7
    NH4Cl 26.8
    MgCl2 37.1
    CaCl2 1.1
    FeCl3 8.11
    Na2-EDTA 4.84
    ZnSO4 2.30
    H3BO3 1.11
    MnCl2.4H2O 0.51
    CoCl2 0.17
    CuSO5.5H2O 0.15
    (NH4)6M07O24.4H2O 0.1
      Suspend 17.5 gram powdered medium in 1 litre distilled water Store dry at room temperature Adjust to pH 6.5
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  • SKU Pack Size Price
    VL60102 1kg $86.63
    VL60103 6 x 1kg $389.81
    VL60101 250g $25.99
    VL6 is a complex medium for cultivation of Discoideum dictyostelium based on Vegetable Peptone. Formedium Ltd have recognised the need for a range of meat-free products. This has led to the development of VL6, an animal component free alternative medium compared to traditional Dicty media such as HL5 and HL5C. VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source.  Yeast Extract is a source of vitamins, co-factors and carbohydrates. A Phosphate buffer is present to inhibit acidification of the medium during cell growth. As most vegetable peptones contain almost no Tryptophane VL6 is supplemented with an extra quantity of this amino acid and some other amino acids. Essential trace elements and vitamins as present in FM and SIH media are added for those Dicty cultures needing additional nutritionals for starting up vigorous growth or protein synthesis.
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    Suspend 35.95g in 1L of Distilled or De-ionised Water
    Components
     
    Vegetable Peptone 10 g/L
    Yeast extract 7 g/L
    Potassium Dihydrogen Phosphate 1.2 g/L
    Disodium Hydrogen Phosphate 0.35 g/L
    Glucose 12 g/L
    Trace elements 0.1 g/L
    VL6 Amino acid supplement 5.3 g/L
    35.95
      The materials used in the production are in compliance with the European Directive 75/318/EEC as amended by Directive 1999/82/EC. Therefore Formedium takes the position that this product is free of any risk in terms of Bovine Spongiform Encephalothy (BSE) or Transmissibe Spongiform Encephalopathy (TSE). HYGROSCOPIC Store at Room Temperature DO NOT BREATHE DUST Keep Container Tightly Closed  
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  • SKU Pack Size Price
    VL60202 1kg $103.95
    VL60203 6 x 1kg $561.33
    VL60201 250g $31.19
    VL6 is a complex medium for cultivation of Discoideum dictyostelium based on Vegetable Peptone. Formedium Ltd have recognised the need for a range of meat-free products. This has led to the development of VL6, an animal component free alternative medium compared to traditional Dicty media such as HL5 and HL5C. VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source. Yeast Extract is a source of vitamins, co-factors and carbohydrates. A Phosphate buffer is present to inhibit acidification of the medium during cell growth. As most vegetable peptones contain almost no Tryptophane VL6 is supplemented with an extra quantity of this amino acid and some other amino acids. Essential trace elements and vitamins as present in FM and SIH media are added for those Dicty cultures needing additional nutritionals for starting up vigorous growth or protein synthesis.
    Select options
    Suspend 23.95g in 1L of Distilled or De-ionised Water
    Components
     
    Vegetable Peptone 10 g/L
    Yeast extract 7 g/L
    Potassium Dihydrogen Phosphate 1.2 g/L
    Disodium Hydrogen Phosphate 0.35 g/L
    Trace elements 0.1 g/L
    VL6 Amino acid supplement 5.3 g/L
    23.95
      The materials used in the production are in compliance with the European Directive 75/318/EEC as amended by Directive 1999/82/EC. Therefore Formedium takes the position that this product is free of any risk in terms of Bovine Spongiform Encephalothy (BSE) or Transmissibe Spongiform Encephalopathy (TSE). HYGROSCOPIC Store at Room Temperature DO NOT BREATHE DUST Keep Container Tightly Closed  
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