VL6 Medium Research

VL6 is a complex medium for cultivation of Discoideum dyctyostelium based on Vegetable Peptone.

Despite a strict policy of sourcing from countries where BSE is not known, and tight regulation and certification of all raw materials, ForMedium Ltd have recognised the need for a range of meat-free products. This has led to the development of VL6 a meat-free alternative medium compared to traditional Dicty media such as HL5 and HL5C.

VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source.  Yeast Extract is a source of vitamins, co-factors and carbohydrates. A Phosphate buffer is present to inhibit acidification of the medium during cell growth.

As most vegetable peptones contain almost no Tryptophane VL6 is supplemented with an extra quantity of this amino acid and some other amino acids. Essential trace elements and vitamins as present in FM and SIH media are added for those Dicty cultures needing additional nutritionals for starting up vigorous growth or protein synthesis.

Components g/l
Vegetable Peptone 10
Yeast extract 7
Potassium Dihydrogen Phosphate 1.2
Disodium Hydrogen Phosphate 0.35
Glucose 12
Trace elements 0.1
VL6 Amino acid supplement 5.3
  35.95

Comparative studies with regards to cell growth of Discoideum dyctyostelium in VL6 and HL5 medium has shown is difference between both media. Results show clearly cell growth ofDiscoideum dyctyostelium in VL6 is as good as in HL5 which is one of the most commonly used non-defined complex medium for culturing dicty.

P for difference +glucose repeat 1 P <0.001; repeat 2 P <0.0001.

24h-timepoints for cell density x 104cells/ml (from standardised 5×104starting concentration).These are data combined from all biological repeats. Temp 22 °C Shaking 180 rpm Vol flasks 15 ml culture.

Usually Vegetable peptones have a lower Nitrogen content compared to animal sourced peptones resulting in a lower rated cell growth. By selecting a nitrogen rich vegetable peptone and the addition of VL6 Amino acid supplement ForMedium has composed a guaranteed BSE and TSE medium with a growth rate equal to HL5. In those cases where the use of BSE and TSE free medium for culturing Discoideum dyctyostelium is required VL6 has proven to be a good alternative.

All materials used in the production are in compliance with the European Directive 75/318/EEC as amended by Directive 1999/82/EC.

Therefore ForMedium Ltd. Takes the position that this product is free of any risk in terms of Bovine Spongiform Encephalothy (BSE) or Transmissibe Spongiform Encephalopathy

VL6 Medium including Glucose GBP £
250 gram 15.00
1 kilogram 50.00
6 x 1 kilogram 225.00

 

VL6 Medium without Glucose GBP £
250 gram 18.00
1 kilogram 60.00
6 x 1 kilogram 324.00

 

Growth data courtesy of BT Ferrara, A Paleckyte and EP Thompson
School of Science, University of Greenwich.

2018-06-15T20:48:06+00:00