Formedium’s YNB mentioned in Subcellular potassium and sodium distribution in Saccharomyces cerevisiae wild-type and vacuolar mutants.

Rito Herrera, María C. Álvarez, Samuel Gelis, José Ramos

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Yeast strains and culture conditions

The S. cerevisiae wild-type and isogenic mutant strains used in the present study and their genotypes are listed in Table 1. Yeast cells were cultured with shaking at 28°C. ‘Normal K+ cells’ were grown in YPD medium [1% (w/v) yeast extract and 2% (w/v) glucose], ‘Na+-grown cells’ were grown in YPD supplemented with 0.8 M NaCl, and ‘K+-starved cells’ were obtained by growing cells in synthetic K+-free medium [YNB-F (0.17% yeast nitrogen base without amino acids, ammonium sulfate and K+ (pH 5.8), Formedium™] supplemented with 50 mM KCl and incubating them for an additional 4 h in the same medium lacking added K+ [18]. Cells were generally harvested during the exponential growth phase at D600 values close to 1.0. In the case of nhx1 mutant grown in Na+, cells were collected at D600 values of 0.2–0.3.

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We thank L. Yenush and J. Ariño for critical comments, D. Canadell and J. Ariño for sharing information on intracellular polyphosphates, and the Translucent Consortium for many useful discussions. We also thank S. Demyda, F. Calahorro, and the groups of M.M. Malagón and J.M. Villalba for technical advice.