Our YPD medium has been mentioned in the following article: New Insights Regarding Yeast Survival following Exposure to Liposomal Amphotericin B.
Rita Teixeira-Santosa,Elisabete Ricardoa,b,Susana Gomes Guerreiroc,d,Sofia Costa-de-Oliveiraa,b,Acácio Gonçalves Rodriguesa,b,e Cidália Pina-Vaza,b,f
The following is a snippet from the report:
MATERIALS AND METHODS
Yeast strains and growth conditions.A broad range of pathogenic and nonpathogenic yeasts were grown in yeast extract-peptone-dextrose (YPD) liquid medium (1% yeast extract, 2% peptone, 2% dextrose; Formedium, Norfolk, United Kingdom) at 35°C with shaking (150 rpm) until the exponential growth phase (details are presented in Table 1) (16). All of the strains were subjected to antifungal susceptibility testing, cell viability assays, and membrane potential evaluation. For membrane integrity, metabolic activity, reactive oxygen species (ROS) production, and apoptotic assays, only Saccharomyces cerevisiae BY4741 was used as a model organism. Prior to experiments, yeasts were subcultured twice in YPD agar to ensure the purity of cultures.
This work was supported by Gilead Sciences grant IN-PT-131-0305.
We are grateful to Catarina Prista from Instituto Superior de Agronomia, Technical University of Lisbon, Portugal, for kindly providing strains, to Vera Machado and Raquel Soares from the Department of Biochemistry, Faculty of Medicine, University of Porto, Portugal, for their help in TUNEL assays, and to Isabel Santos for excellent technical assistance.