Saccharomyces Cerevisiae Yeast Nitrogen Base without Amino Acids

Yeast Nitrogen Base media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.

Besides the original formulation of YNB, ForMedium™produces many variations of this medium without one or more components. These YNB without X media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component

* Please note all formulations within this product range are based upon Yeast Nitrogen Base without Amino Acids and without the Component/Components selected.

 
Product Range
Yeast Nitrogen Base without Amino Acids
without para-Amino Benzoic acid
without Biotin
without Boron
without Calcium
without Calcium-pantothenate
without Copper
without Copper and without Iron
without Folic acid
without Folic acid and Riboflavine. LoFlo
without Inositol
without Iodine
without Iron
without Magnesium
without Manganese
without Molybdene
without Nicotinic acid
without Phosphate
without Phosphate, supplemented with KCl
without Pyridoxine
without Riboflavine
without Sodium
without Thiamine
without Zinc
 
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Yeast Nitrogen Base without Amino acids
 

Yeast Nitrogen Base without amino acids (YNB w/o AA) is used for selecting yeasts based on amino acid and carbohydrate requirements. In Yeast Nitrogen Base with out amino acids Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells.

Compared to full Yeast Nitrogen Base medium, Yeast Nitrogen Base without amino acids lacks Histidine, Methionine and Trypthophan. In combination with Drop Out medium supplement mixtures  Yeast Nitrogen Base without Amino acids provides an excellent medium to cultivate and to select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins. 

The addition of a carbon source like glucose, galactose or raffinose is required. Synthetic Dextrose Minimal medium (SD medium) and Synthetic Complete medium (SC medium) are based upon Yeast Nitrogen Base w/o amino acids and are complemented with glucose, galactose and raffinose as carbon sources.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36
 

 Reference Number Pack Size

GBP (£)

 CYN0401 100 g 18.20
 CYN0402 250 g 39.60
 CYN0405 500 g 80.60
 CYN0410 1000 g 147.30
     
 

Yeast Nitrogen Base without Amino acids and without para-Amino Benzoic acid
 

Para-Amino benzoic acid is omitted from this formulation of Yeast Nitrogen base w/o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of para-Amino Benzoic acid or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN4101 100 g 18.60
 CYN4102 500 g 82.20
 CYN4110 1000 g 151.00
 

Yeast Nitrogen base without Amino acids and without Biotin
 
Biotin is omitted from this formulation of Yeast Nitrogen base w/o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of biotin or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN3101 100 g 18.60
 CYN3102 500 g 82.20
 CYN3110 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Boron
 

Boric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN1501 100 g 18.60
 CYN1502 500 g 82.20
 CYN1510 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Calcium
 

Calcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element.

For technical data please click here.

Suspend 6.8 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN2501 100 g 18.60
 CYN2502 500 g 82.20
 CYN2510 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Calcium Pantothenate
 

Calcium-pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium-pantothenate or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN3301 100 g 18.60
 CYN3302 500 g 82.20
 CYN3310 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Copper
 

Cupric sulphate, CuSO4.5H2O, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper or to replace this element.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN0901 100 g 18.60
 CYN0902 250 g 37.74
 CYN0905 500 g 82.20
 CYN0910 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids, without Copper and without Iron
 

Cupric sulphate, CuSO4.5H2O, and Ferric chloride, FeCl3 are omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper and iron or to replace these elements.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN1301 100 g 18.60
 CYN1302 500 g 82.20
 CYN1310 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Folic Acid
 

Folic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Folic acid or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN3501 100 g 18.60
 CYN3502 500 g 82.20
 CYN3510 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Folic Acid and Riboflavin. LoFlo
 

Yeast Nitrogen Base w/o Amino acids LoFlo is a low fluorescence YNB type medium.

By omitting Folic acid and Riboflavine from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium. 

Yeast Nitrogen Base w/o Amino acids LoFlo is excellent for  whole-cell fluorescence experiments and because of its low fluorescence background the fluorescence of cells can be measured directly in the medium.

Yeast Nitrogen Base w/o Amino acids LoFlo is well suited for intracellular pH measurements in cells expressing pHluorin in combination with Raman spectroscopy.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN6501 100 g 18.60
 CYN6502 250 g 39.60
 CYN6505 500 g 80.60
 CYN6510 1000 g 147.30
     
 

Yeast Nitrogen base without Amino acids and without Inositol
 

Inositol is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of inositol or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN3701 100 g 18.60
 CYN3702 500 g 82.20
 CYN3710 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Iodine
 

Potassium Iodide, KI, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iodine or to replace this element.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN4901 100 g 18.60
 CYN4902 500 g 82.20
 CYN4910 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Iron
 

Ferric chloride, FeCl3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iron or to replace this element.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN1101 100 g 18.60
 CYN1102 500 g 82.20
 CYN1110 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Magnesium
 

Magnesium sulphate, MgSO4.7H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of magnesium or to replace this element.

For technical data please click here.

Suspend 6.2 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN2701 100 g 18.60
 CYN2702 500 g 82.20
 CYN2710 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Manganese
 

Manganese sulphate, MnSO4.H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Manganese or to replace this element.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN1901 100 g 18.60
 CYN1902 500 g 82.20
 CYN1910 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Molybdene
 

Molybdic acid Sodium salt, MoNa2O5.2H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Molybdene or to replace this element.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN2101 100 g 18.60
 CYN2102 500 g 82.20
 CYN2110 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Nicotinic acid
 

Nicotinic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN3901 100 g 18.60
 CYN3902 500 g 82.20
 CYN3910 1000 g 151.00
     
 

Yeast Nitrogen Base without Amino Acids and without Phosphate
 
The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines.

For technical data please click here.

Suspend 5.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36
 

 Reference Number Pack Size

GBP (£)

 CYN0801 100 g 20.80
 CYN0802 250 g 47.30
 CYN0803 500 g 86.00
 CYN0804 1000 g 156.50
     
 

Yeast Nitrogen Base without Amino Acids and without Phosphate, supplemented with KCl
 

The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines.

By omitting Potassium DiHydrogen Phosphate (KH2PO4), not only Phosphate (PO4-3) but also Potassium (K+) is omitted.

As a result yeasts are not only limited in Phosphate, but also in Potassium. This might cause problems in osmo-molarity and intercellular Potassium ion concentrations finally resulting in poor cell growth and low yields.

To avoid this problem an extra 550 mg/l (7.35 mM) of KCl is added. By adding this concentration of KCl the original concentration of K+ as present in YNB is restored. The extra addition of Chlorine is in almost all cases no problems to your cells.

Suspend 6.5 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN6701 100 g 20.80
 CYN6702 250 g 47.30
 CYN6703 500 g 86.00
 CYN6704 1000 g 156.50
     
 

Yeast Nitrogen base without Amino acids and without Pyridoxine
 

Pyridoxine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of pyridoxine or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN4301 100 g 18.60
 CYN4302 500 g 82.20
 CYN4310 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Riboflavine
 

Riboflavine is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN4501 100 g 18.60
 CYN4502 500 g 82.20
 CYN4510 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Sodium
 

Sodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element.

For technical data please click here.

Suspend 6.8 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN2901 100 g 18.60
 CYN2902 500 g 82.20
 CYN2910 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Thiamine
 

Thiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN4701 100 g 18.60
 CYN4702 500 g 82.20
 CYN4710 1000 g 151.00
     
 

Yeast Nitrogen base without Amino acids and without Zinc
 

Zinc sulphate, ZnSO4.7H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Zinc or to replace this element.

For technical data please click here.

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
R: 36/37/38 S: 26,36

 

 Reference Number Pack Size

GBP (£)

 CYN2301 100 g 18.60
 CYN2302 500 g 82.20
 CYN2310 1000 g 151.00
     
 

Technical Data
 
  Yeast Morphology Agar Yeast Nitrogen Base Yeast Potassium Nitrate Nitrogen Base Yeast Nitrogen Base w/o Amino Acids Yeast Nitrogen w/o Amino Acids and w/o Ammonium Sulfate Yeast Carbon Base Vitamin Free Yeast Base
Nitrogen Source g/l              
Ammonium Sulphate 3.5 5 - 5 - - 5
Asparagine 1.5 - - - - -
Potassium Nitrate - - 0.78 - - - -
Caseine Hydrolysate - - - - - - -
Carbon Source g/l              
Glucose.H2O 10 - - - - 10 10
Galactose

-

-

-

-

-

-

-

Raffinose

-

-

-

-

-

-

-

Amino Acids mg/l              
Histidine.HCl

10

10

1

- -

1

10

Methionine

20

20

2

- -

2

20

Tryptophan

20

20

2

- -

2

20

Vitamins g/l              
Biotin

2

2

2

2

2

2

-

Ca-Panthotenate

400

400

400

400

400

400

-

Folic Acid

2

2

2

2

2

2

-

Inositol

2000

2000

2000

2000

2000

2000

-

Nicotinic Acid

400

400

400

400

400

400

-

p-Aminobenzoic Acid

200

200

200

200

200

200

-

Pyridoxine HCl

400

400

400

400

400

400

-

Riboflavin

200

200

200

200

200

200

-

Thiamine HCl

400

400

400

400

400

400

-

Trace Elements g/l  

 

         
Boric Acid

500

500

500

500

500

500

500

Copper Sulfate

40

40

40

40

40

40

40

Potsssium Iodide

100

100

100

100

100

100

100

Ferric Chloride

200

200

200

200

200

200

200

Manganese Sulfate

400

400

400

400

400

400

400

Sodium Molybdate

200

200

200

200

200

200

200

Zinc Sulfate

400

400

400

400

400

400

400

Minerals g/l              
KH2PO4 1 1 1 1 1 1

1

Magnesium Sulphate.anh 0.5 0.5 0.5 0.5 0.5 0.5

0.5

Sodium Chloride 0.1 0.1 0.1 0.1 0.1 0.1

0.1

Calcium Chloride.anh 0.1 0.1 0.1 0.1 0.1 0.1

0.1

Agar g/l              
Agar

18

- - - - - -