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Schizosaccharomyces Pombe Minimal Defined Media Technical Data 2018-01-25T13:55:53+00:00

Schizosaccharomyces Pombe Minimal Defined Media
Technical Data

EMM EMM w/o Dextrose EMM-Low Glucose EMMS
(1,2 M Sorbitol)
EMM w/o Phosphate EMM w/o Nitrogen EMM
SP
MB
Medium
MMA Agar Medium Units
Carbon Source
Dextrose 20.00 5.00 20.00 20.00 20.00 20.00 g
Glucose 5.00 10.00 g
Sorbitol 216.00 g
Amino Acids
Adenine 50.00 mg
Histidine 50.00 mg
Lysine 50.00 mg
L-Leucine 150.00 mg
Uracil 150.00 mg
Minerals
Phtalic Acid K+ 3.00 3.00 3.00 3.00 3.00 3.00 3.00 g
Na2HPO4 2.20 2.20 2.20 2.20 2.20 2.20 g
NH4Cl 5.00 5.00 5.00 5.00 5.00 5.00 g
MgCl2.6H2O 1.05 1.05 1.05 1.05 1.05 1.05 1.05 g
CaCl2.2H2O 14.70 14.70 14.70 14.70 14.70 14.70 14.70 100.00 100.00 mg
KCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 g
Na2SO4 40.00 40.00 40.00 40.00 40.00 40.00 40.00 mg
KH2PO4 500.00 1000.00 mg
K+ Acetate 360.00 mg
MgSO4.7H20 500.00 500.00 mg
NaCl 100.00 100.00 mg
(NH4)2SO4 5000.00 5000.00 mg
Vitamins
Panthotenic acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 mg
Nicotinic acid 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 mg
Inositol 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 mg
Biotin 1.00 1.00 1.00 1.00 1.00 1.00 1.00 0.01 0.01 mg
Ca-Pantothenate 1.00 1.00 mg
Trace Elements
H3BO3 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 mg
MnSO4.H2O 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 mg
ZnSO4.7H2O 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 mg
FeCl3.6H2O 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 mg
Na2MoO4.2H2O 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 mg
KI 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 mg
CuSO4.5H2O 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 mg
Citric Acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 mg
Agar
Agar (17) (17) (17) (17) (17) (17) 17.00 g
Total
Total 32.30 12.30 17.30 248.30 30.10 27.30 32.50 11.90 g/l
Total (Agar) (49.30) (23.30) (34.30) (47.10) (44.30) (49.50) g/l