Para-Amino benzoic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of para-Amino Benzoic acid or to replace this vitamin.
Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.
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Suspend 1.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature