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Yeast Nitrogen Base Media

Saccharomyces Cerevisiae Yeast Nitrogen Base

Yeast Nitrogen Base media have been prepared according to the formulae of Wickerham (1943-51) and Burkholder (1943).  Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB).  YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.

Besides the original formulation of YNB, Formedium™ produces many variations of this medium without one or more components.  These YNB without ‘X’ media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component.

View technical data.

Yeast Nitrogen Base without Amino Acids

Yeast Nitrogen Base without Amino Acids and without Ammonium Sulphate

  • Yeast Carbon Base

    $31.53$255.20
    SKU Pack Size Price
    CYN0601 100g $31.53
    CYN0602 250g $68.61
    CYN0605 500g $139.64
    CYN0610 1000g $255.20
    Yeast Carbon Base without Nitrogen source is used to test the ability of yeasts to assimilate nitrogen by the addition of various nitrogen sources. Histidine, Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base. For technical data please click here. Suspend 11.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • Yeast Morphology Agar

    $47.82$344.42
    SKU Pack Size Price
    CYN0101 100g $47.82
    CYN0102 250g $103.95
    CYN0105 500g $189.19
    CYN0110 1000g $344.42
    Yeast Agar is a rich well defined medium including Ammonium Sulfate and Asparagine as a nitrogen source and is used for typical colonial morphology. The medium provides a carbon- and nitrogen source in sufficient concentrations including amino acids, vitamins, trace elements and salts resulting in a good development of yeast colonies. For technical data please click here. Suspend 35.0 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • Yeast Nitrogen Base

    $30.49$255.20
    SKU Pack Size Price
    CYN0201 100g $30.49
    CYN0202 250g $68.61
    CYN0205 500g $139.64
    CYN0210 1000g $255.20
    Yeast Nitrogen Base is based upon the formulation of Yeast Agar except that Ammonium Sulfate is the sole nitrogen source and that addition of carbon source is required. The medium may be used to test carbon assimilation in the presence of a carbon source like glucose, galactose or raffinose. For technical data please click here. Suspend 6.9 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • SKU Pack Size Price
    CYN0301 100g $43.49
    CYN0302 250g $94.42
    CYN0305 500g $172.04
    CYN0310 1000g $313.06
    Yeast Potassium Nitrate Nitrogen Base contains Potassium Nitrate as a sole nitrogen source to test nitrogen assimilation. The addition of a carbon source is required. Histidine, Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base. Yeasts which have grown on a rich medium like Yeast Agar may carry a reserve of nitrogen in the form of protein. To avoid errors due to this reserve of nitrogen it is advised to make two serial transfers in the medium. Seven days after the first inoculation the second inoculation is made in the same medium. If a positive result is obtained after seven days, the yeast cells assimilates nitrate as sole nitrogen source. For technical data please click here. Suspend 2.7 gram powdered medium in 1 litre distilled water Store dry at room temperature
  • Yeast Vitamin Free Base

    $31.53$255.20
    SKU Pack Size Price
    CYN0701 100g $31.53
    CYN0702 250g $68.61
    CYN0705 500g $139.64
    CYN0710 1000g $255.20
    Yeast Nitrogen Base Vitamin Free is based upon the formulation of Yeast Nitrogen base except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins. For technical data please click here. Suspend 17.0 gram powdered medium in 1 litre distilled water Store dry at room temperature