Formedium’s products mentioned in Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories

Formedium’s YNB without amino acids, CSM-URA and G418 have all been mentioned in an article about the Production of fatty acid-derived oleochemicals and biofules by synthetic yeast cell factories.

The following is a snippet from the report:

Strain cultivation

Yeast strains for preparation of competent cells were cultivated in YPD consisting of 10 g l−1 yeast extract (Merck Millipore), 20 g l−1 peptone (Difco) and 20 g l−1 glucose (Merck Millipore). Strains containing URA3-based plasmids or cassettes were selected on synthetic complete media without uracil (SC-URA), which consisted of 6.7 g l−1 yeast nitrogen base (YNB) without amino acids (Formedium), 0.77 g l−1 complete supplement mixture without uracil (CSM-URA, Formedium), 20 g l−1 glucose (Merck Millipore) and 18 g l−1 agar (Merck Millipore). The URA3 maker was removed and selected against on SC+FOA plates, which contained 6.7 g l−1 YNB, 0.77 g l−1 complete supplement mixture and 0.8 g l−1 5-fluoroorotic acid. Strains containing the kanMX cassettes were selected on YPD plates containing 200 mg l−1 G418 (Formedium).

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Reference:
http://www.nature.com/articles/ncomms11709#t1

– 25th May 2016.

 

2018-03-04T09:56:24+00:00