Formedium HL5-C used in Ag-DNA research

Our HL5-C Medium has been used in a research into Ag-DNA by Nelli Bossert, Donny de Bruin, Maria Götz, Dirk Bouwmeester & Doris Heinrich. The HL5-C Medium was used for Dictyostelium discoideum HG1729 histone-GFP in AX2 and Dictyostelium discoideum Wildtype in AX2 cells cultures.

The following is a snippet from the report:

Cell culture

Dictyostelium discoideum HG1729 histone-GFP in AX2 and Dictyostelium discoideum Wildtype in AX2 cells were cultured in HL5-C medium adjusted to pH 6.7 at 21 °C; medium for HG1729 histone-GFP was supplemented with 10 μg/ml Geneticin. For the experiments the cells were harvested below a confluency of 40% and washed three times with PB by centrifugation at 400 g for 5 min. Subsequently, cells dispersed in PB were seeded onto an observation chamber (composed of a cover glass and a custom-made Teflon® frame) and rested for 30 min, allowing the cells to settle down.

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Reference:
http://www.nature.com/articles/srep37897 Scientific Reports 6, Article number: 37897 (2016). Published online: 30 November 2016, Nelli Bossert, Donny de Bruin, Maria Götz, Dirk Bouwmeester & Doris Heinrich.

2018-03-04T10:04:31+00:00